TY - JOUR
T1 - Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract
AU - Arung, Enos Tangke
AU - Kusuma, Irawan Wijaya
AU - Purwatiningsih, Sri
AU - Roh, Seong Soo
AU - Yang, Chae Ha
AU - Jeon, Soohyeon
AU - Kim, Yong Ung
AU - Sukaton, Edi
AU - Susilo, Joko
AU - Astuti, Yuli
AU - Wicaksono, Britanto Dani
AU - Sandra, Ferry
AU - Shimizu, Kuniyoshi
AU - Kondo, Ryuichiro
N1 - Funding Information:
This work was funded by DIKTI of Indonesian Education Ministry with grant 026/SP2H/PP/DP2M/III/2008 . This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund , KRF-2008-331-E00467) .
PY - 2009/12
Y1 - 2009/12
N2 - We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50μg/mL, 87.5μg/mL, and 125μg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.
AB - We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50μg/mL, 87.5μg/mL, and 125μg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.
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U2 - 10.1016/S2005-2901(09)60073-X
DO - 10.1016/S2005-2901(09)60073-X
M3 - Article
C2 - 20633507
AN - SCOPUS:73049089124
VL - 2
SP - 306
EP - 308
JO - JAMS Journal of Acupuncture and Meridian Studies
JF - JAMS Journal of Acupuncture and Meridian Studies
SN - 2005-2901
IS - 4
ER -
