Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Sonam Tamrakar, Hai Bang Tran, Marina Nishida, Satoru Kaifuchi, Hiroto Suhara, Katsumi Doi, Katsuya Fukami, Gopal Prasad Parajuli, Kuniyoshi Shimizu

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

Mushrooms have garnered immense popularity for their nutritional as well as medicinal values. The therapeutic potential of mushrooms in Nepal, a country well known for its biodiversity and natural medicinal resources, remains largely unstudied. Therefore, this study attempts to unveil the antioxidative properties of Nepalese wild mushrooms. Sixty-two wild mushroom samples were collected from several forests in different parts of Nepal. Ethanol and water extracts of the dried samples were tested for their antioxidative activities using total phenolic content (TPC), oxygen radical absorbance capacity (ORAC) assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power (RP) assays. Ethanol extracts of samples belonging to the order Hymenochaetales showed significantly high activity in all the assays. Inonotus clemensiae had an exceptionally high TPC of 643.2 mg gallic acid equivalent (GAE)/g extract and also exhibited the lowest EC50 values in DPPH (0.081 mg/mL), ABTS (0.409 mg/mL), and EC0.5 value in reducing power (RP; 0.031 mg/mL) assays. High-performance liquid chromatography (HPLC) analysis of the top ten samples with the highest TPC was done to identify the phenolic compounds in the extracts, followed by liquid chromatography–mass spectrometry (LC–MS) analysis for some unknown compounds. These findings highlight the very strong antioxidative activity of Nepalese mushrooms, and paves the way for further research to explore their economic potential.

元の言語英語
ページ(範囲)769-779
ページ数11
ジャーナルJournal of Natural Medicines
70
発行部数4
DOI
出版物ステータス出版済み - 10 1 2016

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Nepal
Agaricales
Assays
Ethanol
Gallic Acid
Sulfonic Acids
Biodiversity
High performance liquid chromatography
Spectrometry
Reactive Oxygen Species
Spectrum Analysis
Economics
High Pressure Liquid Chromatography
Water
Liquids
Research
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

これを引用

Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species. / Tamrakar, Sonam; Tran, Hai Bang; Nishida, Marina; Kaifuchi, Satoru; Suhara, Hiroto; Doi, Katsumi; Fukami, Katsuya; Parajuli, Gopal Prasad; Shimizu, Kuniyoshi.

:: Journal of Natural Medicines, 巻 70, 番号 4, 01.10.2016, p. 769-779.

研究成果: ジャーナルへの寄稿記事

Tamrakar, Sonam ; Tran, Hai Bang ; Nishida, Marina ; Kaifuchi, Satoru ; Suhara, Hiroto ; Doi, Katsumi ; Fukami, Katsuya ; Parajuli, Gopal Prasad ; Shimizu, Kuniyoshi. / Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species. :: Journal of Natural Medicines. 2016 ; 巻 70, 番号 4. pp. 769-779.
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