Antiviral effects against influenza a virus infection by a short hairpin rna targeting the non-coding terminal region of the viral nucleoprotein gene

Yoshikazu Fujimoto, Kenji Kyogoku, Keiko Takeda, Kinuyo Ozaki, Sayo Yamamoto, Haruka Suyama, Etsuro Ono

研究成果: ジャーナルへの寄稿記事

抄録

RNA interference (RNAi) can inhibit Influenza A virus (IAV) infection in a gene-specific manner. In this study, we constructed a transgene expressing a short hairpin RNA (shRNA) that targets the noncoding region of the IAV RNA gene encoding nucleoprotein (NP). To investigate the antiviral effects of the shRNA, we generated two transgenic mouse lines with this transgene. Unfortunately, there was no apparent difference in IAV resistance between transgenic and non-transgenic littermates. To further investigate the antiviral effects of the shRNA, we prepared mouse embryonic fibroblasts (MEFs) from transgenic and non-transgenic mice. In experimental infections using these MEFs, virus production of mouse-adapted IAV strain A/Puerto Rico/8/1934 (PR8) in the transgenic MEFs was suppressed by means of the down-regulation of the viral RNA gene transcription in the early stages of infection in comparison with non-transgenic MEFs. These results indicated that expression of the shRNA was able to confer antiviral properties against IAVs to MEFs, although the effects were limited. Our findings suggest that the shRNA targeting the noncoding region of the viral RNA (vRNA) of NP might be a supporting tool in developing influenza-resistant poultry.

元の言語英語
ページ(範囲)383-388
ページ数6
ジャーナルJournal of Veterinary Medical Science
81
発行部数3
DOI
出版物ステータス出版済み - 3 1 2019

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nucleoproteins
Nucleoproteins
Viral Genes
Virus Diseases
Orthomyxoviridae
Small Interfering RNA
Antiviral Agents
Influenza A virus
small interfering RNA
Fibroblasts
fibroblasts
mice
infection
genes
Viral RNA
genetically modified organisms
Transgenes
Transgenic Mice
RNA
transgenes

All Science Journal Classification (ASJC) codes

  • veterinary(all)

これを引用

Antiviral effects against influenza a virus infection by a short hairpin rna targeting the non-coding terminal region of the viral nucleoprotein gene. / Fujimoto, Yoshikazu; Kyogoku, Kenji; Takeda, Keiko; Ozaki, Kinuyo; Yamamoto, Sayo; Suyama, Haruka; Ono, Etsuro.

:: Journal of Veterinary Medical Science, 巻 81, 番号 3, 01.03.2019, p. 383-388.

研究成果: ジャーナルへの寄稿記事

Fujimoto, Yoshikazu ; Kyogoku, Kenji ; Takeda, Keiko ; Ozaki, Kinuyo ; Yamamoto, Sayo ; Suyama, Haruka ; Ono, Etsuro. / Antiviral effects against influenza a virus infection by a short hairpin rna targeting the non-coding terminal region of the viral nucleoprotein gene. :: Journal of Veterinary Medical Science. 2019 ; 巻 81, 番号 3. pp. 383-388.
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