Apaf-1-independent programmed cell death in mouse development

A. Nagasaka, K. Kawane, H. Yoshida, S. Nagata

研究成果: ジャーナルへの寄稿学術誌査読

48 被引用数 (Scopus)


Many cells die during mammalian development and are engulfed by macrophages. In DNase II -/- embryos, the TUNEL-positive DNA of apoptotic cells is left undigested in macrophages, providing a system for studying programmed cell death during mouse development. Here, we showed that an Apaf-1-null mutation in the DNase II -/- embryos greatly reduced the number of macrophages carrying DNA at E11.5. However, at later stages of the embryogenesis, a significant number of macrophages carrying undigested DNA were present in Apaf-1 -/- embryos, indicating that cells died and were engulfed in an Apaf-1-independent manner. In most tissues of the Apaf-1 -/- embryos, no processed caspase-3 was detected, and the DNA of dead cells accumulated in the macrophages appeared intact. Many nonapoptotic dead cells were found in the tail of the Apaf-1 -/- embryos, suggesting that the Apaf-1-independent programmed cell death occurred, and these dead cells were engulfed by macrophages. In contrast, active caspase-3 was detected in E14.5 thymus of Apaf-1 -/- embryos. Treatment of fetal thymocytes with staurosporine, but not etoposide, induced processing of procaspases 3 and 9, indicating that the E14.5 thymocytes have the ability to undergo caspase-dependent apoptosis in an Apaf-1-independent manner. Thus, programmed cell death in mouse development, which normally proceeds in an efficient Apaf-1-depenent mechanism, appears to be backed up by Apaf-1-independent death systems.

ジャーナルCell Death and Differentiation
出版ステータス出版済み - 6月 2010

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学


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