Apical membrane and junctional complex formation during simple epithelial cell differentiation of F9 cells

Satoshi Komiya, Masayuki Shimizu, Junichi Ikenouchi, Shigenobu Yonemura, Takeshi Matsui, Yoshitaka Fukunaga, Huijie Liu, Fumio Endo, Shoichiro Tsukita, Akira Nagafuchi

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

Epithelium formation is a common event in animal morphogenesis. It has been reported that F9 cells differentiate into visceral endoderm-like epithelial cells when cell aggregates are cultured in the presence of retinoic acid. The present investigation set out to determine whether this in vitro model could be used under monolayer culture conditions, which is suitable for a detailed analysis of epithelial differentiation. We performed comparative gene expression analyses of F9 cells grown under aggregate and monolayer culture conditions prior to and following treatment with retinoic acid. Under these conditions, induction in the expression of differentiation marker genes was confirmed, even in monolayer cultures. Junctional complex and apical membrane formation, both of which are characteristic of epithelial cells, were also observed under monolayer culture conditions. Because of the merit of monolayer culture condition, we found that apical membrane and junctional complex formation are strictly regulated during epithelial differentiation. It was also revealed that F9 cells differentiated into epithelial cells predominantly on the fourth and fifth day following retinoic acid induction. These results showed that a monolayer culture of F9 cells represents a viable in vitro model that can be employed to elucidate mechanisms pertaining to epithelium formation.

本文言語英語
ページ(範囲)1065-1080
ページ数16
ジャーナルGenes to Cells
10
11
DOI
出版ステータス出版済み - 11 2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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