Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives - inter-laboratory evaluation study

Tomoko Shimamura, Yoshihiro Sumikura, Takeshi Yamazaki, Atsuko Tada, Takehiro Kashiwagi, Hiroya Ishikawa, Toshiro Matsui, Naoki Sugimoto, Hiroshi Akiyama, Hiroyuki Ukeda

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抜粋

An inter-laboratory evaluation study was conducted in order to evaluate the antioxidant capacity of food additives by using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Four antioxidants used as existing food additives (i.e., tea extract, grape seed extract, enju extract, and d-α-tocopherol) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were used as analytical samples, and 14 laboratories participated in this study. The repeatability relative standard deviation (RSD r ) of the IC 50 of Trolox, four antioxidants, and the Trolox equivalent antioxidant capacity (TEAC) were 1.8 - 2.2%, 2.2 - 2.9%, and 2.1 - 2.5%, respectively. Thus, the proposed DPPH assay showed good performance within the same laboratory. The reproducibility relative standard deviation (RSD R ) of IC 50 of Trolox, four antioxidants, and TEAC were 4.0 - 7.9%, 6.0 - 11%, and 3.7 - 9.3%, respectively. The RSD R /RSD r values of TEAC were lower than, or nearly equal to, those of IC 50 of the four antioxidants, suggesting that the use of TEAC was effective for reducing the variance among the laboratories. These results showed that the proposed DPPH assay could be used as a standard method to evaluate the antioxidant capacity of food additives.

元の言語英語
ページ(範囲)717-721
ページ数5
ジャーナルanalytical sciences
30
発行部数7
DOI
出版物ステータス出版済み - 2014

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

フィンガープリント Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives - inter-laboratory evaluation study' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Shimamura, T., Sumikura, Y., Yamazaki, T., Tada, A., Kashiwagi, T., Ishikawa, H., Matsui, T., Sugimoto, N., Akiyama, H., & Ukeda, H. (2014). Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives - inter-laboratory evaluation study. analytical sciences, 30(7), 717-721. https://doi.org/10.2116/analsci.30.717