Application of 2-amino-6-vinylpurine as an efficient agent for conjugation of oligonucleotides

Md Monsur Ali, Fumi Nagatsugi, Shigeki Sasaki, Ryusuke Nakahara, Minoru Maeda

研究成果: Contribution to journalArticle

8 引用 (Scopus)

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Attempts have been made to conjugate a variety of molecules with oligonucleotides to achieve useful functions. In this study, we have established a new efficient method for post-synthetic conjugation of oligonucleotides with the use of the 2-amino-6-vinylpurine nucleoside. Amino nucleophiles form the corresponding conjugates under acidic conditions, whereas thiol nucleophiles reacted efficiently under alkaline conditions. Thus, glutathione and HS-Cys-(Arg)8 without protecting groups were efficiently conjugated to the 2-amino-6-vinylpurine-bearing ODN under alkaline conditions. The use of 2-amino-6-vinylpurine as an agent for conjugation is advantageous in that it is stable during the reaction and may be applied to conjugation of ODNs with multiple functional molecules.

元の言語英語
ページ(範囲)159-169
ページ数11
ジャーナルNucleosides, Nucleotides and Nucleic Acids
25
発行部数2
DOI
出版物ステータス出版済み - 1 1 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Genetics

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