Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice

Yuji Ueda, Koichi Kawamoto, Masamitsu Konno, Kozo Noguchi, Satoru Kaifuchi, Taroh Satoh, Hidetoshi Eguchi, Yuichiro Doki, Takaaki Hirotsu, Masaki Mori, Hideshi Ishii

研究成果: ジャーナルへの寄稿記事

抄録

Pancreatic ductal adenocarcinoma (PDAC) exhibits a very early onset of metastasis. Thus, early detection and treatment are pivotal to successful eradication of pancreatic cancers. Economical and non-invasive cancer screening systems is indispensable for this purpose. Previously our group developed a novel method to detect various kinds of human cancer using nematode Caenorhabditis elegans (C. elegans) that respond to cancer odor in urine; however, whether this method is useful for non-human species remains to be understood. In this study, we examined its effectiveness in the detection of murine pancreatic tumor spontaneously generated in genetically-engineered mice. We generated pancreas-specific KrasG12D and/or c-Met deletion mutant mice and measured the probability of spontaneous tumor generation in these mice. The chemotactic indexes of C. elegans to the urine samples of these mutant mice were measured. As previously described, oncogenic KrasG12D was necessary to induce pancreatic intraepithelial neoplasia in this mouse model, while c-Met mutation did not show further effect. The chemotactic analysis indicated that C. elegans avoids urine of healthy recipient mice, while they tended to be attracted to urine of mice with KrasG12D. Our study demonstrated that C. elegans can recognize the odor of pancreatic cancer in urine of KrasG12D model mouse, suggesting the similarity of cancer odor between species. Our result facilitates further studies on mechanism of cancer detection by C. elegans.

元の言語英語
ページ(範囲)5412-5418
ページ数7
ジャーナルOncotarget
10
発行部数52
出版物ステータス出版済み - 1 1 2019

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Caenorhabditis elegans
Early Detection of Cancer
Urine
Neoplasms
Pancreatic Neoplasms
Pancreas
Adenocarcinoma
Neoplasm Metastasis
Mutation

All Science Journal Classification (ASJC) codes

  • Oncology

これを引用

Ueda, Y., Kawamoto, K., Konno, M., Noguchi, K., Kaifuchi, S., Satoh, T., ... Ishii, H. (2019). Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice. Oncotarget, 10(52), 5412-5418.

Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice. / Ueda, Yuji; Kawamoto, Koichi; Konno, Masamitsu; Noguchi, Kozo; Kaifuchi, Satoru; Satoh, Taroh; Eguchi, Hidetoshi; Doki, Yuichiro; Hirotsu, Takaaki; Mori, Masaki; Ishii, Hideshi.

:: Oncotarget, 巻 10, 番号 52, 01.01.2019, p. 5412-5418.

研究成果: ジャーナルへの寄稿記事

Ueda, Y, Kawamoto, K, Konno, M, Noguchi, K, Kaifuchi, S, Satoh, T, Eguchi, H, Doki, Y, Hirotsu, T, Mori, M & Ishii, H 2019, 'Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice', Oncotarget, 巻. 10, 番号 52, pp. 5412-5418.
Ueda Y, Kawamoto K, Konno M, Noguchi K, Kaifuchi S, Satoh T その他. Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice. Oncotarget. 2019 1 1;10(52):5412-5418.
Ueda, Yuji ; Kawamoto, Koichi ; Konno, Masamitsu ; Noguchi, Kozo ; Kaifuchi, Satoru ; Satoh, Taroh ; Eguchi, Hidetoshi ; Doki, Yuichiro ; Hirotsu, Takaaki ; Mori, Masaki ; Ishii, Hideshi. / Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice. :: Oncotarget. 2019 ; 巻 10, 番号 52. pp. 5412-5418.
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abstract = "Pancreatic ductal adenocarcinoma (PDAC) exhibits a very early onset of metastasis. Thus, early detection and treatment are pivotal to successful eradication of pancreatic cancers. Economical and non-invasive cancer screening systems is indispensable for this purpose. Previously our group developed a novel method to detect various kinds of human cancer using nematode Caenorhabditis elegans (C. elegans) that respond to cancer odor in urine; however, whether this method is useful for non-human species remains to be understood. In this study, we examined its effectiveness in the detection of murine pancreatic tumor spontaneously generated in genetically-engineered mice. We generated pancreas-specific KrasG12D and/or c-Met deletion mutant mice and measured the probability of spontaneous tumor generation in these mice. The chemotactic indexes of C. elegans to the urine samples of these mutant mice were measured. As previously described, oncogenic KrasG12D was necessary to induce pancreatic intraepithelial neoplasia in this mouse model, while c-Met mutation did not show further effect. The chemotactic analysis indicated that C. elegans avoids urine of healthy recipient mice, while they tended to be attracted to urine of mice with KrasG12D. Our study demonstrated that C. elegans can recognize the odor of pancreatic cancer in urine of KrasG12D model mouse, suggesting the similarity of cancer odor between species. Our result facilitates further studies on mechanism of cancer detection by C. elegans.",
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