Application of diffusion ordered-1H-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages

Ruge Cao, Airi Nonaka, Fusae Komura, Toshiro Matsui

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)


This work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative 1H-nuclear magnetic resonance spectroscopy (DOSY-qNMR), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of sucrose in deuterium oxide at 30 °C was 4.9 × 10-10m2/s at field gradient pulse from 5.0 × 10-2to 3.0 × 10-1T/m, separated from other carbohydrates (glucose and fructose). Good linearity (r2= 0.9999) was obtained between sucrose (0.5-20.0 g/L) and the resonance area of target glucopyranosyl-α-C1 proton normalised to that of cellobiose C1 proton (100.0 g/L, as an internal standard) in 1D sliced DOSY spectrum. The DOSY-qNMR method was successfully applied to quantify sucrose in orange juice (36.1 ± 0.5 g/L), pineapple juice (53.5 ± 1.1 g/L) and a sports drink (24.7 ± 0.6 g/L), in good agreement with the results obtained by an F-kit method.

ジャーナルFood Chemistry
出版ステータス出版済み - 3 15 2015

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 食品科学


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