抄録
Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 83-88 |
| ページ数 | 6 |
| ジャーナル | Journal of the Faculty of Agriculture, Kyushu University |
| 巻 | 43 |
| 発行部数 | 1-2 |
| 出版物ステータス | 出版済み - 11 1998 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences (miscellaneous)
これを引用
Application of Flow Cytometry for Rapid Determination of Ploidy Levels in Asparagus (Asparagus officinalis L.). / Ozaki, Yukio; Narikiyo, Kumiko; Hiramatsu, Michikazu; Ureshino, Kenji; Okubo, Hiroshi.
：: Journal of the Faculty of Agriculture, Kyushu University, 巻 43, 番号 1-2, 11.1998, p. 83-88.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Application of Flow Cytometry for Rapid Determination of Ploidy Levels in Asparagus (Asparagus officinalis L.)
AU - Ozaki, Yukio
AU - Narikiyo, Kumiko
AU - Hiramatsu, Michikazu
AU - Ureshino, Kenji
AU - Okubo, Hiroshi
PY - 1998/11
Y1 - 1998/11
N2 - Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
AB - Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
UR - http://www.scopus.com/inward/record.url?scp=0032268156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032268156&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032268156
VL - 43
SP - 83
EP - 88
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 1-2
ER -