Application of high Tc SQUID magnetometer to biological immunoassays

Keiji Enpuku, T. Minotani, M. Hotta, A. Nakahodo

研究成果: ジャーナルへの寄稿Conference article

58 引用 (Scopus)

抄録

A high Tc SQUID system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. Design and set up of the system is described. Minimum detectable amplitude of the magnetic flux is 0.6 mΦ0 for the measurement bandwidth from 0.2 Hz to 5 Hz when we use a magnetometer. The system noise does not increase when the magnetic field of 0.8 mT is applied in parallel to the SQUID. An experiment to measure the antigen-antibody reaction shows that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. When a gradiometer is used, the system noise decreased by a factor of 5, compared to the case of the magnetometer. This improvement indicates the usefulness of the gradiometer to suppress the residual environmental noise in the present system. Magnetic markers that have remanent magnetic moment are also studied in order to increase the signal.

元の言語英語
ページ(範囲)661-664
ページ数4
ジャーナルIEEE Transactions on Applied Superconductivity
11
発行部数1 I
DOI
出版物ステータス出版済み - 3 1 2001
イベント2000 Applied Superconductivity Conference - Virginia Beach, VA, 米国
継続期間: 9 17 20009 22 2000

Fingerprint

immunoassay
SQUIDs
Magnetometers
markers
magnetometers
gradiometers
Antigen-antibody reactions
antigens
antibodies
Magnetic flux
Antigens
Magnetic moments
Antibodies
Magnetic fields
Nanoparticles
Bandwidth
magnetic flux
magnetic moments
bandwidth
nanoparticles

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

これを引用

Application of high Tc SQUID magnetometer to biological immunoassays. / Enpuku, Keiji; Minotani, T.; Hotta, M.; Nakahodo, A.

:: IEEE Transactions on Applied Superconductivity, 巻 11, 番号 1 I, 01.03.2001, p. 661-664.

研究成果: ジャーナルへの寄稿Conference article

Enpuku, Keiji ; Minotani, T. ; Hotta, M. ; Nakahodo, A. / Application of high Tc SQUID magnetometer to biological immunoassays. :: IEEE Transactions on Applied Superconductivity. 2001 ; 巻 11, 番号 1 I. pp. 661-664.
@article{d1cbb5f4ba854a45904d0fc0be72e38b,
title = "Application of high Tc SQUID magnetometer to biological immunoassays",
abstract = "A high Tc SQUID system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. Design and set up of the system is described. Minimum detectable amplitude of the magnetic flux is 0.6 mΦ0 for the measurement bandwidth from 0.2 Hz to 5 Hz when we use a magnetometer. The system noise does not increase when the magnetic field of 0.8 mT is applied in parallel to the SQUID. An experiment to measure the antigen-antibody reaction shows that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. When a gradiometer is used, the system noise decreased by a factor of 5, compared to the case of the magnetometer. This improvement indicates the usefulness of the gradiometer to suppress the residual environmental noise in the present system. Magnetic markers that have remanent magnetic moment are also studied in order to increase the signal.",
author = "Keiji Enpuku and T. Minotani and M. Hotta and A. Nakahodo",
year = "2001",
month = "3",
day = "1",
doi = "10.1109/77.919431",
language = "English",
volume = "11",
pages = "661--664",
journal = "IEEE Transactions on Applied Superconductivity",
issn = "1051-8223",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "1 I",

}

TY - JOUR

T1 - Application of high Tc SQUID magnetometer to biological immunoassays

AU - Enpuku, Keiji

AU - Minotani, T.

AU - Hotta, M.

AU - Nakahodo, A.

PY - 2001/3/1

Y1 - 2001/3/1

N2 - A high Tc SQUID system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. Design and set up of the system is described. Minimum detectable amplitude of the magnetic flux is 0.6 mΦ0 for the measurement bandwidth from 0.2 Hz to 5 Hz when we use a magnetometer. The system noise does not increase when the magnetic field of 0.8 mT is applied in parallel to the SQUID. An experiment to measure the antigen-antibody reaction shows that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. When a gradiometer is used, the system noise decreased by a factor of 5, compared to the case of the magnetometer. This improvement indicates the usefulness of the gradiometer to suppress the residual environmental noise in the present system. Magnetic markers that have remanent magnetic moment are also studied in order to increase the signal.

AB - A high Tc SQUID system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. Design and set up of the system is described. Minimum detectable amplitude of the magnetic flux is 0.6 mΦ0 for the measurement bandwidth from 0.2 Hz to 5 Hz when we use a magnetometer. The system noise does not increase when the magnetic field of 0.8 mT is applied in parallel to the SQUID. An experiment to measure the antigen-antibody reaction shows that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. When a gradiometer is used, the system noise decreased by a factor of 5, compared to the case of the magnetometer. This improvement indicates the usefulness of the gradiometer to suppress the residual environmental noise in the present system. Magnetic markers that have remanent magnetic moment are also studied in order to increase the signal.

UR - http://www.scopus.com/inward/record.url?scp=0035268586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035268586&partnerID=8YFLogxK

U2 - 10.1109/77.919431

DO - 10.1109/77.919431

M3 - Conference article

AN - SCOPUS:0035268586

VL - 11

SP - 661

EP - 664

JO - IEEE Transactions on Applied Superconductivity

JF - IEEE Transactions on Applied Superconductivity

SN - 1051-8223

IS - 1 I

ER -