Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed

Mitsuru Miyanoshita, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.

元の言語英語
ページ(範囲)2014-2017
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
79
発行部数12
DOI
出版物ステータス出版済み - 1 1 2015

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Ribonuclease P
Nucleic Acid Hybridization
Nucleic Acids
DNA
Fluorescence Resonance Energy Transfer
Proteins
Assays
Nucleotides
Annealing

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

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