Arteriolar niches maintain haematopoietic stem cell quiescence

Yuya Kunisaki, Ingmar Bruns, Christoph Scheiermann, Jalal Ahmed, Sandra Pinho, Dachuan Zhang, Toshihide Mizoguchi, Qiaozhi Wei, Daniel Lucas, Keisuke Ito, Jessica C. Mar, Aviv Bergman, Paul S. Frenette

研究成果: ジャーナルへの寄稿記事

542 引用 (Scopus)

抜粋

Cell cycle quiescence is a critical feature contributing to haematopoietic stem cell (HSC) maintenance. Although various candidate stromal cells have been identified as potential HSC niches, the spatial localization of quiescent HSCs in the bone marrow remains unclear. Here, using a novel approach that combines whole-mount confocal immunofluorescence imaging techniques and computational modelling to analyse significant three-dimensional associations in the mouse bone marrow among vascular structures, stromal cells and HSCs, we show that quiescent HSCs associate specifically with small arterioles that are preferentially found in endosteal bone marrow. These arterioles are ensheathed exclusively by rare NG2 (also known as CSPG4) + pericytes, distinct from sinusoid-associated leptin receptor (LEPR) + cells. Pharmacological or genetic activation of the HSC cell cycle alters the distribution of HSCs from NG2 + periarteriolar niches to LEPR + perisinusoidal niches. Conditional depletion of NG2 + cells induces HSC cycling and reduces functional long-term repopulating HSCs in the bone marrow. These results thus indicate that arteriolar niches are indispensable for maintaining HSC quiescence.

元の言語英語
ページ(範囲)637-643
ページ数7
ジャーナルNature
502
発行部数7473
DOI
出版物ステータス出版済み - 10 10 2013
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • General

これを引用

Kunisaki, Y., Bruns, I., Scheiermann, C., Ahmed, J., Pinho, S., Zhang, D., ... Frenette, P. S. (2013). Arteriolar niches maintain haematopoietic stem cell quiescence. Nature, 502(7473), 637-643. https://doi.org/10.1038/nature12612