抄録
The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) activity in aromatic hydrocarbon (Ah) responsive and non-responsive strains of mice were studied using the hepatic microsomal fraction. Injection of 3-methylcholanthrene (MC; 42 mg kg body wt.) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 120 μg kg body wt.) into both strains produced marked enhancement of AHH activity except for MC treatment of Ah non-responsive strains. Addition of 7,8-benzoflavone (BNF) to the microsomal AHH assay mixture prepared from mice previously injected with vehicle (olive oil) alone caused an increase in activity when the mice were responsive, while BNF lowered the activity in non-responsive strains. With regard to MC-injected mice, BNF and 3-methyl-sulphonyl-4,5,3',4'-tetrachlorobiphenyl (3-MSF-TCB) decreased microsomal AHH activity in Ah-responsive mice, whereas these drugs enhanced the activity in Ah-non-responsive strains. 3-MSF-TCB also had inhibitory potency on AHH activity, but the mechanism of inhibition seems to be somewhat different from that of BNF. It may also suggest that cytochrome P-450 isozymes inhibited by BNF are different from those inhibited by 3-MSF-TCB.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 73-80 |
| ページ数 | 8 |
| ジャーナル | Toxicology Letters |
| 巻 | 52 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1990 |
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All Science Journal Classification (ASJC) codes
- Toxicology
これを引用
Aryl hydrocarbon hydroxylase activity levels in the hepatic microsomal fraction from MC- or TCDD-treated mice : a comparison between aromatic hydrocarbon responsive and non-responsive strains. / Kiyohara, Chikako; Mohri, N.; Hirohata, T.; Haraguchi, K.; Masuda, Y.; Nagayama, J.
：: Toxicology Letters, 巻 52, 番号 1, 01.01.1990, p. 73-80.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Aryl hydrocarbon hydroxylase activity levels in the hepatic microsomal fraction from MC- or TCDD-treated mice
T2 - a comparison between aromatic hydrocarbon responsive and non-responsive strains
AU - Kiyohara, Chikako
AU - Mohri, N.
AU - Hirohata, T.
AU - Haraguchi, K.
AU - Masuda, Y.
AU - Nagayama, J.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) activity in aromatic hydrocarbon (Ah) responsive and non-responsive strains of mice were studied using the hepatic microsomal fraction. Injection of 3-methylcholanthrene (MC; 42 mg kg body wt.) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 120 μg kg body wt.) into both strains produced marked enhancement of AHH activity except for MC treatment of Ah non-responsive strains. Addition of 7,8-benzoflavone (BNF) to the microsomal AHH assay mixture prepared from mice previously injected with vehicle (olive oil) alone caused an increase in activity when the mice were responsive, while BNF lowered the activity in non-responsive strains. With regard to MC-injected mice, BNF and 3-methyl-sulphonyl-4,5,3',4'-tetrachlorobiphenyl (3-MSF-TCB) decreased microsomal AHH activity in Ah-responsive mice, whereas these drugs enhanced the activity in Ah-non-responsive strains. 3-MSF-TCB also had inhibitory potency on AHH activity, but the mechanism of inhibition seems to be somewhat different from that of BNF. It may also suggest that cytochrome P-450 isozymes inhibited by BNF are different from those inhibited by 3-MSF-TCB.
AB - The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) activity in aromatic hydrocarbon (Ah) responsive and non-responsive strains of mice were studied using the hepatic microsomal fraction. Injection of 3-methylcholanthrene (MC; 42 mg kg body wt.) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 120 μg kg body wt.) into both strains produced marked enhancement of AHH activity except for MC treatment of Ah non-responsive strains. Addition of 7,8-benzoflavone (BNF) to the microsomal AHH assay mixture prepared from mice previously injected with vehicle (olive oil) alone caused an increase in activity when the mice were responsive, while BNF lowered the activity in non-responsive strains. With regard to MC-injected mice, BNF and 3-methyl-sulphonyl-4,5,3',4'-tetrachlorobiphenyl (3-MSF-TCB) decreased microsomal AHH activity in Ah-responsive mice, whereas these drugs enhanced the activity in Ah-non-responsive strains. 3-MSF-TCB also had inhibitory potency on AHH activity, but the mechanism of inhibition seems to be somewhat different from that of BNF. It may also suggest that cytochrome P-450 isozymes inhibited by BNF are different from those inhibited by 3-MSF-TCB.
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U2 - 10.1016/0378-4274(90)90167-K
DO - 10.1016/0378-4274(90)90167-K
M3 - Article
VL - 52
SP - 73
EP - 80
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 1
ER -