ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation

Keiji Uematsu, Fumihiko Okumura, Syunsuke Tonogai, Akiko Joo-Okumura, Dawit Hailu Alemayehu, Akihiko Nishikimi, Yoshinori Fukui, Kunio Nakatsukasa, Takumi Kamura

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Proper dynamic regulation of the spindle is essential for successful cell division. However, the molecular mechanisms that regulate spindle dynamics in mitosis are not fully understood. In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics, thereby targeting it for proteasomal degradation. The presence of microtubules (MTs) prevented the ASB7-DDA3 interaction, thus stabilizing DDA3. Knockdown of ASB7 decreased MT polymerization and increased the proportion of cells with unaligned chromosomes, and this phenotype was rescued by deletion of DDA3. Collectively, these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3.

元の言語英語
記事番号95
ジャーナルJournal of Cell Biology
215
発行部数1
DOI
出版物ステータス出版済み - 1 1 2016

Fingerprint

Microtubules
Suppressor of Cytokine Signaling Proteins
Cullin Proteins
Genome
Mitosis
Polymerization
Cell Division
Chromosomes
Phenotype

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. / Uematsu, Keiji; Okumura, Fumihiko; Tonogai, Syunsuke; Joo-Okumura, Akiko; Alemayehu, Dawit Hailu; Nishikimi, Akihiko; Fukui, Yoshinori; Nakatsukasa, Kunio; Kamura, Takumi.

:: Journal of Cell Biology, 巻 215, 番号 1, 95, 01.01.2016.

研究成果: ジャーナルへの寄稿記事

Uematsu, K, Okumura, F, Tonogai, S, Joo-Okumura, A, Alemayehu, DH, Nishikimi, A, Fukui, Y, Nakatsukasa, K & Kamura, T 2016, 'ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation', Journal of Cell Biology, 巻. 215, 番号 1, 95. https://doi.org/10.1083/jcb.201603062
Uematsu, Keiji ; Okumura, Fumihiko ; Tonogai, Syunsuke ; Joo-Okumura, Akiko ; Alemayehu, Dawit Hailu ; Nishikimi, Akihiko ; Fukui, Yoshinori ; Nakatsukasa, Kunio ; Kamura, Takumi. / ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. :: Journal of Cell Biology. 2016 ; 巻 215, 番号 1.
@article{8bcb93f13c0f45fa8dbf1d8ef3903257,
title = "ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation",
abstract = "Proper dynamic regulation of the spindle is essential for successful cell division. However, the molecular mechanisms that regulate spindle dynamics in mitosis are not fully understood. In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics, thereby targeting it for proteasomal degradation. The presence of microtubules (MTs) prevented the ASB7-DDA3 interaction, thus stabilizing DDA3. Knockdown of ASB7 decreased MT polymerization and increased the proportion of cells with unaligned chromosomes, and this phenotype was rescued by deletion of DDA3. Collectively, these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3.",
author = "Keiji Uematsu and Fumihiko Okumura and Syunsuke Tonogai and Akiko Joo-Okumura and Alemayehu, {Dawit Hailu} and Akihiko Nishikimi and Yoshinori Fukui and Kunio Nakatsukasa and Takumi Kamura",
year = "2016",
month = "1",
day = "1",
doi = "10.1083/jcb.201603062",
language = "English",
volume = "215",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation

AU - Uematsu, Keiji

AU - Okumura, Fumihiko

AU - Tonogai, Syunsuke

AU - Joo-Okumura, Akiko

AU - Alemayehu, Dawit Hailu

AU - Nishikimi, Akihiko

AU - Fukui, Yoshinori

AU - Nakatsukasa, Kunio

AU - Kamura, Takumi

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Proper dynamic regulation of the spindle is essential for successful cell division. However, the molecular mechanisms that regulate spindle dynamics in mitosis are not fully understood. In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics, thereby targeting it for proteasomal degradation. The presence of microtubules (MTs) prevented the ASB7-DDA3 interaction, thus stabilizing DDA3. Knockdown of ASB7 decreased MT polymerization and increased the proportion of cells with unaligned chromosomes, and this phenotype was rescued by deletion of DDA3. Collectively, these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3.

AB - Proper dynamic regulation of the spindle is essential for successful cell division. However, the molecular mechanisms that regulate spindle dynamics in mitosis are not fully understood. In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics, thereby targeting it for proteasomal degradation. The presence of microtubules (MTs) prevented the ASB7-DDA3 interaction, thus stabilizing DDA3. Knockdown of ASB7 decreased MT polymerization and increased the proportion of cells with unaligned chromosomes, and this phenotype was rescued by deletion of DDA3. Collectively, these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3.

UR - http://www.scopus.com/inward/record.url?scp=84991250600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991250600&partnerID=8YFLogxK

U2 - 10.1083/jcb.201603062

DO - 10.1083/jcb.201603062

M3 - Article

C2 - 27697924

AN - SCOPUS:84991250600

VL - 215

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

M1 - 95

ER -