Assembly of MreB filaments on liposome membranes: A synthetic biology approach

Yusuke T. Maeda, Tomoyoshi Nakadai, Jonghyeon Shin, Kunihiro Uryu, Vincent Noireaux, Albert Libchaber

研究成果: ジャーナルへの寄稿学術誌査読

88 被引用数 (Scopus)

抄録

The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4-10 μm rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.

本文言語英語
ページ(範囲)53-59
ページ数7
ジャーナルACS Synthetic Biology
1
2
DOI
出版ステータス出版済み - 2月 17 2012
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生体医工学
  • 生化学、遺伝学、分子生物学(その他)

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