Assessment of genetic diversity in Egyptian barley (Hordeum vulgare L.) genotypes using SSR and SNP markers

Ammar Elakhdar, Toshihiro Kumamaru, Calvin O. Qualset, Robert S. Brueggeman, Khairy Amer, Ludovic Capo-chichi

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

A barley core collection can be studied extensively and the derived information can be used to identify loci/genes for the genetic improvement of quantitative and qualitative traits. To assess genetic diversity, allelic variation and population structure of Egyptian barley, 134 barley genotypes collected from a different region along with 19 cultivated genotypes obtained from of the Egyptian Agricultural Research Center. All genotypes were analyzed with 261 polymorphic SSR and SNP alleles. The mean number of alleles per locus was 4 and PIC was 0.49, while the level of genetic diversity was 0.55 ranging from 0.03 to 0.82. The genotypes were assigned to three subpopulations that were consistent with their origins. The genetic variation within population was higher (51%) than among population at the molecular levels (FST = 0.491 when P < 0.10). The level of polymorphic variation was highest in subpopulations-II, due to collected from different regions with different ear-types thus, expected to contain more diversity than local genotypes in subpopulations-I and subpopulations-III. The structured study found that the 153 barley genotypes are in harmony with clustering approaches using the SSR and SNP genotypic data in a neighbor-joining tree and principal components analysis, which identified three subpopulations. These results demonstrated genetic diversity among the Egyptian barley genotypes can be applied to suggest approaches, such as association analysis, classical mapping population development, and parental line selection in breeding programs. Therefore, it is necessary to use the exotic genotypes as the genetic resources for developing new barley cultivars in Egypt.

元の言語英語
ページ(範囲)1937-1951
ページ数15
ジャーナルGenetic Resources and Crop Evolution
65
発行部数7
DOI
出版物ステータス出版済み - 10 1 2018

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Hordeum
barley
Hordeum vulgare
Single Nucleotide Polymorphism
genotype
Genotype
subpopulation
genetic variation
Population
allele
Alleles
alleles
loci
Genetic Loci
marker
genetic diversity
genetic resource
Egypt
agricultural research
Principal Component Analysis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

これを引用

Assessment of genetic diversity in Egyptian barley (Hordeum vulgare L.) genotypes using SSR and SNP markers. / Elakhdar, Ammar; Kumamaru, Toshihiro; Qualset, Calvin O.; Brueggeman, Robert S.; Amer, Khairy; Capo-chichi, Ludovic.

:: Genetic Resources and Crop Evolution, 巻 65, 番号 7, 01.10.2018, p. 1937-1951.

研究成果: ジャーナルへの寄稿記事

Elakhdar, Ammar ; Kumamaru, Toshihiro ; Qualset, Calvin O. ; Brueggeman, Robert S. ; Amer, Khairy ; Capo-chichi, Ludovic. / Assessment of genetic diversity in Egyptian barley (Hordeum vulgare L.) genotypes using SSR and SNP markers. :: Genetic Resources and Crop Evolution. 2018 ; 巻 65, 番号 7. pp. 1937-1951.
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