Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10

Karla J. Matteson, James Picado-Leonard, Bon Chu Chung, T. K. Mohandas, Walter L. Miller

研究成果: ジャーナルへの寄稿記事

127 引用 (Scopus)

抄録

P450cl7 is the single enzyme mediating both 17α-hydroxylase and 17,20 lyase activities. We identified several human P450cl7 cDNA clones in a human adrenal cDNA library we constructed in XgtlO. A short clone containing the 3’-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450cl7 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450cl7 gene lies on chromosome 10. The chromosomal locations of the other P450cl7 genes could not be determined.

元の言語英語
ページ(範囲)789-791
ページ数3
ジャーナルJournal of Clinical Endocrinology and Metabolism
63
発行部数3
DOI
出版物ステータス出版済み - 9 1986

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Steroid 17-alpha-Hydroxylase
Chromosomes, Human, Pair 10
Human Chromosomes
Chromosomes
Genes
Steroids
Hybrid Cells
Complementary DNA
Clone Cells
DNA
DNA Restriction Enzymes
Cell Line
Mixed Function Oxygenases
Gene Library
Human Genome
Southern Blotting
Cells
Enzymes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

これを引用

Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10. / Matteson, Karla J.; Picado-Leonard, James; Chung, Bon Chu; Mohandas, T. K.; Miller, Walter L.

:: Journal of Clinical Endocrinology and Metabolism, 巻 63, 番号 3, 09.1986, p. 789-791.

研究成果: ジャーナルへの寄稿記事

Matteson, Karla J. ; Picado-Leonard, James ; Chung, Bon Chu ; Mohandas, T. K. ; Miller, Walter L. / Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10. :: Journal of Clinical Endocrinology and Metabolism. 1986 ; 巻 63, 番号 3. pp. 789-791.
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abstract = "P450cl7 is the single enzyme mediating both 17α-hydroxylase and 17,20 lyase activities. We identified several human P450cl7 cDNA clones in a human adrenal cDNA library we constructed in XgtlO. A short clone containing the 3’-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450cl7 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450cl7 gene lies on chromosome 10. The chromosomal locations of the other P450cl7 genes could not be determined.",
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AU - Picado-Leonard, James

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AU - Mohandas, T. K.

AU - Miller, Walter L.

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AB - P450cl7 is the single enzyme mediating both 17α-hydroxylase and 17,20 lyase activities. We identified several human P450cl7 cDNA clones in a human adrenal cDNA library we constructed in XgtlO. A short clone containing the 3’-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450cl7 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450cl7 gene lies on chromosome 10. The chromosomal locations of the other P450cl7 genes could not be determined.

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