抄録
In Xenopus laevis embryos a high concentration of both KCl and 0.5% DOC (sodium deoxycholate) is needed for maximal extraction of ribosomes and polysomes. We studied the nature of the structures that keep ribosomes and polysomes immobilized within the cytoplasm of embryonic cells at cleavage through tailbud stages, using various combinations of a low‐salt buffer (20 mM KCl), a high‐salt buffer (500 mM KCl), 0.5% DOC, and 0.5% Triton X‐100. With a low‐salt buffer and 0.5% DOC, but not Triton X‐100, 80S ribosomal monomers and polysomes were liberated from the cytoplasmic rapidly sedimenting structures (RSS) to the soluble fraction. With a high‐salt buffer (500 mM KCl), ribosomes were solubilized as 60S and 40S subunits together with about one‐half of the total polysomes. When cells were homogenized in a low‐salt buffer with added inhibitors of the cytoskeleton (cytochalasin B or colchicine), the majority of polysomes but not ribosomes were solubilized. These results provide evidence for the following conclusions. 1) Polysomes are bound to cytoskeletal structures in Xenopus embryos, but ribosomes, both maternal and newly synthesized, are associated with membranous noncytoskeletal structures. 2) The membranous structures consist of two compartments, one high‐salt sensitive and the other high‐salt resistant. 3) Ribosomes of the high‐salt resistant group increase in amount with developmental stage and appear to be the precursor to the ribosomes of the high‐salt sensitive group.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 227-236 |
| ページ数 | 10 |
| ジャーナル | Journal of Experimental Zoology |
| 巻 | 235 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1985 |
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All Science Journal Classification (ASJC) codes
- Animal Science and Zoology
これを引用
Association of maternal and newly synthesized ribosomes with membranous noncytoskeletal structures in Xenopus laevis embryonic cells. / Shiokawa, Koichiro; Tashiro, Kosuke; Misumi, Yoshio.
：: Journal of Experimental Zoology, 巻 235, 番号 2, 01.01.1985, p. 227-236.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Association of maternal and newly synthesized ribosomes with membranous noncytoskeletal structures in Xenopus laevis embryonic cells
AU - Shiokawa, Koichiro
AU - Tashiro, Kosuke
AU - Misumi, Yoshio
PY - 1985/1/1
Y1 - 1985/1/1
N2 - In Xenopus laevis embryos a high concentration of both KCl and 0.5% DOC (sodium deoxycholate) is needed for maximal extraction of ribosomes and polysomes. We studied the nature of the structures that keep ribosomes and polysomes immobilized within the cytoplasm of embryonic cells at cleavage through tailbud stages, using various combinations of a low‐salt buffer (20 mM KCl), a high‐salt buffer (500 mM KCl), 0.5% DOC, and 0.5% Triton X‐100. With a low‐salt buffer and 0.5% DOC, but not Triton X‐100, 80S ribosomal monomers and polysomes were liberated from the cytoplasmic rapidly sedimenting structures (RSS) to the soluble fraction. With a high‐salt buffer (500 mM KCl), ribosomes were solubilized as 60S and 40S subunits together with about one‐half of the total polysomes. When cells were homogenized in a low‐salt buffer with added inhibitors of the cytoskeleton (cytochalasin B or colchicine), the majority of polysomes but not ribosomes were solubilized. These results provide evidence for the following conclusions. 1) Polysomes are bound to cytoskeletal structures in Xenopus embryos, but ribosomes, both maternal and newly synthesized, are associated with membranous noncytoskeletal structures. 2) The membranous structures consist of two compartments, one high‐salt sensitive and the other high‐salt resistant. 3) Ribosomes of the high‐salt resistant group increase in amount with developmental stage and appear to be the precursor to the ribosomes of the high‐salt sensitive group.
AB - In Xenopus laevis embryos a high concentration of both KCl and 0.5% DOC (sodium deoxycholate) is needed for maximal extraction of ribosomes and polysomes. We studied the nature of the structures that keep ribosomes and polysomes immobilized within the cytoplasm of embryonic cells at cleavage through tailbud stages, using various combinations of a low‐salt buffer (20 mM KCl), a high‐salt buffer (500 mM KCl), 0.5% DOC, and 0.5% Triton X‐100. With a low‐salt buffer and 0.5% DOC, but not Triton X‐100, 80S ribosomal monomers and polysomes were liberated from the cytoplasmic rapidly sedimenting structures (RSS) to the soluble fraction. With a high‐salt buffer (500 mM KCl), ribosomes were solubilized as 60S and 40S subunits together with about one‐half of the total polysomes. When cells were homogenized in a low‐salt buffer with added inhibitors of the cytoskeleton (cytochalasin B or colchicine), the majority of polysomes but not ribosomes were solubilized. These results provide evidence for the following conclusions. 1) Polysomes are bound to cytoskeletal structures in Xenopus embryos, but ribosomes, both maternal and newly synthesized, are associated with membranous noncytoskeletal structures. 2) The membranous structures consist of two compartments, one high‐salt sensitive and the other high‐salt resistant. 3) Ribosomes of the high‐salt resistant group increase in amount with developmental stage and appear to be the precursor to the ribosomes of the high‐salt sensitive group.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021827623&partnerID=8YFLogxK
U2 - 10.1002/jez.1402350209
DO - 10.1002/jez.1402350209
M3 - Article
C2 - 4056690
AN - SCOPUS:0021827623
VL - 235
SP - 227
EP - 236
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
SN - 0022-104X
IS - 2
ER -