Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation

Izuho Hatada, Masakazu Namihira, Sumiyo Morita, Mika Kimura, Takuro Horii, Kinichi Nakashima

研究成果: ジャーナルへの寄稿学術誌査読

65 被引用数 (Scopus)

抄録

Epigenetic changes are though to lead to alterations in the property of cells, such as differentiation potential. Neural precursor cells (NPCs) differentiate only into neurons in the midgestational brain, yet they become able to generate astrocytes in the late stage of development. This differentiation-potential switch could be explained by epigenetic changes, since the promoters of astrocyte-specific marker genes, glial fibrillary acidic protein (Gfap) and S100β, have been shown to become demethylated in late-stage NPCs prior to the onset of astrocyte differentiation; however, whether demethylation occurs generally in other astrocyctic genes remains unknown. Here we analyzed DNA methylation changes in mouse NPCs between the mid-(E11.5) and late (E14.5) stage of development by a genome-wide DNA methylation profiling method using microarrays and found that many astrocytic genes are demethylated in late-stage NPCs, enabling the cell to become competent to express these genes. Although these genes are already demethytated in late-staqe NPCs, they are not expressed until cells differentiate into astrocytes. Thus, late-stage NPCs have epigenetic potential which can be realized in their expression after astrocyte differentiation.

本文言語英語
論文番号e3189
ジャーナルPloS one
3
9
DOI
出版ステータス出版済み - 9月 11 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

フィンガープリント

「Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル