Ca2+ transport system in the intracellular membranes was studied by using saponin-treated macrophages of the guinea pig, in which the plasma membranes could be selectively destroyed. Saponin-treated macrophages could accumulate 3.1 nmoles Ca2+ 4 × 106 macrophages in the presence of Mg-ATP and sodium azide with an apparent affinity constant of 6 × 106 M-1. In the absence of sodium azide, the value of Ca2+ uptake of saponin-treated macrophages was 95 nmoles/4 × 106 macrophages, and its affinity constant for Ca2+ was 3 × 105 M-1. Saponin-treated macrophages may be suitable for the studies of Ca2+ transport systems in the intracellular membranes.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||出版済み - 2月 26 1982|
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