ATP-dependent Ca2+ accumulation in intracellular membranes of guinea pig macrophages after saponin treatment

Masato Hirata, Toshitaka Koga

研究成果: ジャーナルへの寄稿学術誌査読

26 被引用数 (Scopus)

抄録

Ca2+ transport system in the intracellular membranes was studied by using saponin-treated macrophages of the guinea pig, in which the plasma membranes could be selectively destroyed. Saponin-treated macrophages could accumulate 3.1 nmoles Ca2+ 4 × 106 macrophages in the presence of Mg-ATP and sodium azide with an apparent affinity constant of 6 × 106 M-1. In the absence of sodium azide, the value of Ca2+ uptake of saponin-treated macrophages was 95 nmoles/4 × 106 macrophages, and its affinity constant for Ca2+ was 3 × 105 M-1. Saponin-treated macrophages may be suitable for the studies of Ca2+ transport systems in the intracellular membranes.

本文言語英語
ページ(範囲)1544-1549
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
104
4
DOI
出版ステータス出版済み - 2月 26 1982

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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