ATP-gated current in dissociated rat nucleus solitarii neurons

S. Ueno, N. Harata, K. Inoue, N. Akaike

研究成果: Contribution to journalArticle査読

91 被引用数 (Scopus)

抄録

1. The excitatory response of extracellularly applied ATP was investigated in freshly dissociated rat nucleus tractus solitarii neurons under whole-cell configuration using the 'concentration-clamp' technique. 2. At a holding potential of -70 mV, 100 μM ATP evoked inward current that was slowly desensitized in the continuous presence of ATP. The ATP-gated current increased in a concentration-dependent manner over the concentration range between 10 μM and 1 mM. The half-maximum concentration was 31 μM and the Hill coefficient was 1.2. 3. The potency of ATP analogues for the purinergic receptor was in the order of ATP = 2-methylthio-ATP >> ADP > α,β-methylene ATP. Neither adenosine nor AMP evoked any responses. The order was consistent with a P(2y) receptor subtype. 4. The current-voltage relationship for the 100 μM ATP response showed a clear inward rectification at positive potentials beyond -50 mV. The reversal potential of the ATP-gated current was +13 mV. 5. The time constants of activation and inactivation of the ATP-gated current solution were dependent on the extracellular ATP concentration, and both kinetics became faster at higher ATP concentrations. 6. The ATP-gated current was also elicited in an external solution containing Ca2+ as a permeable cation. The inactivation kinetics in an external solution containing 75 mM Ca2+ were faster than those in an external solution with 150 mM Na+. 7. Calculated relative permeability ratios were P(Na)/P(Cs) = 1.64 ([Na+](o) = 30-150 mM), P(Ca)/P(Cs) = 2.17 ([Ca2+](o) = 2 mM). Anions were not measurably permeable in this preparation.

本文言語英語
ページ(範囲)778-785
ページ数8
ジャーナルJournal of Neurophysiology
68
3
DOI
出版ステータス出版済み - 1 1 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 生理学

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