Augmented expression of LCK message directed from the downstream promoter in human colorectal cancer specimens

Kazuhiko Nakamura, Y. Chijiiwa, H. Nawata

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Lck protein is expressed in some colon carcinoma cell lines but its expression in colon cancer cells in vivo has not been clarified. LCK transcription is regulated from two distinct promoters and initiated exclusively from the downstream promoter in colon carcinoma cell lines in contrast to peripheral lymphocytes. We investigated the expression of the downstream promoter-initiated LCK transcript in 18 colorectal primary cancer and normal mucosae, and two hepatic metastases, using a RNase protection assay with the EcoRI-BglII fragment of human LCK cDNA, YT16. In normal tissues, only traces of the LCK transcript were detected. The expression of the LCK transcript was augmented in 3/18 cancer specimens. The relative level of the LCK transcript in the cancer tissue compared to the average value of normal adjacent tissue was 10-60 in 3 cases, and 3-10 in 7 cases. One hepatic metastasis expressed more LCK message than the primary lesion. Our results indicate that the LCK message is strongly expressed in some colorectal cancers.

元の言語英語
ページ(範囲)1401-1407
ページ数7
ジャーナルEuropean Journal of Cancer Part A
32
発行部数8
DOI
出版物ステータス出版済み - 1 1 1996

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Colorectal Neoplasms
Colon
Neoplasm Metastasis
Carcinoma
Cell Line
Liver
Ribonucleases
Colonic Neoplasms
Neoplasms
Reference Values
Mucous Membrane
Complementary DNA
Lymphocytes
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Augmented expression of LCK message directed from the downstream promoter in human colorectal cancer specimens. / Nakamura, Kazuhiko; Chijiiwa, Y.; Nawata, H.

:: European Journal of Cancer Part A, 巻 32, 番号 8, 01.01.1996, p. 1401-1407.

研究成果: ジャーナルへの寄稿記事

Nakamura, Kazuhiko ; Chijiiwa, Y. ; Nawata, H. / Augmented expression of LCK message directed from the downstream promoter in human colorectal cancer specimens. :: European Journal of Cancer Part A. 1996 ; 巻 32, 番号 8. pp. 1401-1407.
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N2 - Lck protein is expressed in some colon carcinoma cell lines but its expression in colon cancer cells in vivo has not been clarified. LCK transcription is regulated from two distinct promoters and initiated exclusively from the downstream promoter in colon carcinoma cell lines in contrast to peripheral lymphocytes. We investigated the expression of the downstream promoter-initiated LCK transcript in 18 colorectal primary cancer and normal mucosae, and two hepatic metastases, using a RNase protection assay with the EcoRI-BglII fragment of human LCK cDNA, YT16. In normal tissues, only traces of the LCK transcript were detected. The expression of the LCK transcript was augmented in 3/18 cancer specimens. The relative level of the LCK transcript in the cancer tissue compared to the average value of normal adjacent tissue was 10-60 in 3 cases, and 3-10 in 7 cases. One hepatic metastasis expressed more LCK message than the primary lesion. Our results indicate that the LCK message is strongly expressed in some colorectal cancers.

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