Automated chemiluminescence immunoassay for a nonionic surfactant using a recycled spinning-pausing controlled washing procedure on a compact disc-type microfluidic platform

Shuai Guo, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    研究成果: ジャーナルへの寄稿学術誌査読

    10 被引用数 (Scopus)

    抄録

    A fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (CD)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (APnEOs) is described. The pattern of the CD-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the CD-type microchip. The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area. The detection limit of the immunoassay is about 10 ppb.

    本文言語英語
    ページ(範囲)100-106
    ページ数7
    ジャーナルTalanta
    133
    DOI
    出版ステータス出版済み - 2月 2015

    !!!All Science Journal Classification (ASJC) codes

    • 分析化学

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