Automated determination of drugs in serum by column-switching high-performance liquid chromatography. IV. Separation of tricyclic and tetracyclic antidepressants and their metabolites

K. Matsumoto, S. Kanba, H. Kubo, G. Yagi, H. Iri, H. Yuki

研究成果: Contribution to journalArticle査読

29 被引用数 (Scopus)

抄録

We describe automated column-switching high-performance liquid chromatography for determining nine tricyclic and tetracyclic antidepressants (TCAs) and their metabolites in human serum. TSKgel ODS-80TM and TSKprecolumn PW (Tosoh Co., Tokyo) are used in the analytical column and the precolumn, respectively. A 200-μL serum sample is directly injected onto the precolumn. After washing the serum proteins from the precolumn with potassium phosphate buffer, the precolumn connection is switched to introduce the retained substances onto the analytical column. The drugs are then eluted within 30 min with an acetonitrile/potassium phosphate buffer mixture containing sodium 1-heptanesulfonate. The analytical recoveries (95-104%), reproducibilities (within-run CV <3%), and detection limits (10 μg/L) indicate that this HPLC system is suited for therapeutic drug monitoring. Correlations were good between the TCA concentrations in serum and administered dose (r = 0.713, n = 41), and between 10-hydroxynortriptyline and nortriptyline in serum (r = 0.691, n = 24).

本文言語英語
ページ(範囲)453-456
ページ数4
ジャーナルClinical Chemistry
35
3
DOI
出版ステータス出版済み - 1989

All Science Journal Classification (ASJC) codes

  • 臨床生化学
  • 生化学、医学

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