Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma

Mamoru Harada, Goro Matsuzaki, Yasunobu Yoshikai, Noritada Kobayashi, Shin Kurosawa, Hiroaki Takimoto, Kikuo Nomoto

研究成果: Contribution to journalArticle査読

34 被引用数 (Scopus)


A CD4+ heat shock protein (hsp) 60-recognizing autoreactive T-ceil line (BASL1) and clone (BASL1.1) were examined for their antitumor activity against major histocompatibility complex class II” syngeneic Meth A fibrosarcoma (Meth A), which was immunofluorescently stained with monoclonal antibody specific for hsp 60. In in vitro proliferative assay, BASL1.1 was suggested to recognize Meth A-derived hsp 60 presented by syngeneic antigen-presenting cells in a major histocompatibility complex class II-restricted manner. This cell line and clone showed antitumor activity in tumor-neutralizing (Winn) assay. BASL1 and BASL1.1 cells produced y-interferon, tumor necrosis factor, and interleukin 2 but not interleukin 4 by the stimulation with syngeneic spleen cells. In cytolytic assay, these cell lines and clones showed neither direct nor indirect (bystander) cytol-ysis against Meth A. In cytostatic assay, these cells inhibited the proliferation of Meth A in the presence of syngeneic macrophages, and this activity was abrogated by the addition of anti-y-interferon monoclonal antibody. Recombinant γ-interferon could induce cytostatic activity only in the presence of macrophages, and tumor necrosis factor synergized this activity. Antitumor activity induced by BASL1 was abrogated by the administration of anti-CD8 monoclonal antibody in vivo, suggesting that CD8+ cytotoxic T-lymphocytes are essential and final effector cells for BASLl-mediated Meth A rejection. These findings indicate that CD4+ autoreactive and hsp 60-recognizing T-cells show two types of antitumor activity: cytostasis and induction of tumor-specific cytotoxic T-lymphocytes. Furthermore, these results imply that tumor-specific immunity could be elicited by CD4+ helper T-cells which recognize hsp.

ジャーナルCancer Research
出版ステータス出版済み - 1 1993

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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