Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation

Miho Hayashi, Yoshitoshi Ogura, Elizabeth J. Harry, Naotake Ogasawara, Shigeki Moriya

研究成果: ジャーナルへの寄稿学術誌査読

35 被引用数 (Scopus)

抄録

It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.

本文言語英語
ページ(範囲)73-79
ページ数7
ジャーナルFEMS microbiology letters
247
1
DOI
出版ステータス出版済み - 6月 1 2005
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学

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