Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics

Zhenhao Ding, Hiromichi Kasahara, Michihiko Nakano, Junya Suehiro

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

In this study, an electrical DNA detection method was applied to bacterial detection. DNA was extracted from bacteria and amplified by polymerase chain reaction. The microbeads were labelled with amplicons, altering their surface conductance and therefore their dielectrophoresis characteristics. Amplicon-labelled microbeads could thus be trapped within a high-strength electric field, where they formed a pearl chain between the electrodes, resulting in an increased conductance between the electrodes. This method reduces the amplicon detection time from 1-2 h to 15 min, compared with the conventional method. The presented method realised quantitative detection of specific bacteria at concentrations above 1×105 and 2.4 × 104 CFU/ml for bacterial solutions with and without other bacterial presence, respectively.

本文言語英語
ページ(範囲)562-567
ページ数6
ジャーナルIET Nanobiotechnology
11
5
DOI
出版ステータス出版済み - 8 1 2017

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • バイオテクノロジー
  • 電子工学および電気工学

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