TY - JOUR
T1 - Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters
AU - Lee, Jae Man
AU - Xu, Jian
AU - Mon, Hiroaki
AU - Mitsudome, Takumi
AU - Masuda, Atsushi
AU - Yoshimura, Kaito
AU - Iiyama, Kazuhiro
AU - Chieda, Yuuka
AU - Kusakabe, Takahiro
N1 - Funding Information:
This work was supported in part by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Integrated research project for plant, insect and animal using genome technology INSECT-1201), and KAKENHI nos. 22248003 and 23580077 from the Japan Society for the Promotion of Science . The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University .
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/3
Y1 - 2014/3
N2 - Baculoviruses serve as efficient viral vectors for gene delivery into vertebrate and invertebrate cells. The identification and characterization of the functional promoters in different baculovirus-infected hosts are essential for the efficient gene expression. To establish a baculovirus-mediated gene transfer system in the silkworm, Bombyx mori, we investigated the activities of silkworm-derived TCTP, ACTIN3, and HSC70-4 promoters delivered by AcNPV or BmNPV in various tissues of silkworm. In many of the tested silkworm tissues, the BmHSC70-4 promoter exhibited a higher transcription activity than those of BmTCTP or BmACTIN3 promoters when delivered by AcNPV, which is reported to be incapable of replicating in silkworms. In contrast, the BmACTIN3 promoter was found to be the strongest promoters when delivered by BmNPV. The present results indicate that the BmHSC70-4 promoter is potentially useful for the stable gene expression by the non-replicating AcNPV vector for gene function analysis in the silkworm.
AB - Baculoviruses serve as efficient viral vectors for gene delivery into vertebrate and invertebrate cells. The identification and characterization of the functional promoters in different baculovirus-infected hosts are essential for the efficient gene expression. To establish a baculovirus-mediated gene transfer system in the silkworm, Bombyx mori, we investigated the activities of silkworm-derived TCTP, ACTIN3, and HSC70-4 promoters delivered by AcNPV or BmNPV in various tissues of silkworm. In many of the tested silkworm tissues, the BmHSC70-4 promoter exhibited a higher transcription activity than those of BmTCTP or BmACTIN3 promoters when delivered by AcNPV, which is reported to be incapable of replicating in silkworms. In contrast, the BmACTIN3 promoter was found to be the strongest promoters when delivered by BmNPV. The present results indicate that the BmHSC70-4 promoter is potentially useful for the stable gene expression by the non-replicating AcNPV vector for gene function analysis in the silkworm.
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U2 - 10.1016/j.aspen.2013.10.009
DO - 10.1016/j.aspen.2013.10.009
M3 - Article
AN - SCOPUS:84896970131
VL - 17
SP - 73
EP - 78
JO - Journal of Asia-Pacific Entomology
JF - Journal of Asia-Pacific Entomology
SN - 1226-8615
IS - 1
ER -