Baicalein Inhibits Stx1 and 2 of EHE: Effects of Baicalein on the Cytotoxicity, Production, and Secretion of Shiga Toxins of Enterohaemorrhagic Escherichia coli

Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Hoang Minh Duc, Motokazu Nakayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken Ichi Honjoh, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿記事

抄録

Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen. Baicalein (5,6,7-trihydroxylflavone), a flavone isolated from the roots of Scutellaria baicalensis, is considered as a potential antibacterial agent to control foodborne pathogens. Among seven compounds selected by in silico screening of the natural compound database, baicalein inhibited the cytotoxicity of both Shiga toxins 1 and 2 (Stx1 and Stx2) against Vero cells after pretreatment at 0.13 mmol/L. In addition, baicalein reduced the susceptibility of Vero cells to both Stx1 and Stx2. Real-time qPCR showed that baicalein increased transcription of stx1 but not of stx2. However, baicalein had no effects on production or secretion of Stx1 or Stx2. Docking models suggested that baicalein formed a stable structure with StxB pentamer with low intramolecular energy. The results demonstrate that inhibitory activity of baicalein against the cytotoxicity of both Stx1 and Stx2 might be due to of the formation of a binding structure inside the pocket of the Stx1B and Stx2B pentamers.

元の言語英語
ジャーナルToxins
11
発行部数9
DOI
出版物ステータス出版済み - 8 29 2019

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Shiga Toxins
Enterohemorrhagic Escherichia coli
Cytotoxicity
Escherichia coli
flavone
Vero Cells
Pathogens
Shiga Toxin 1
Shiga Toxin 2
Scutellaria baicalensis
Shiga Toxin
Shiga-Toxigenic Escherichia coli
Escherichia coli O157
Transcription
baicalein
Computer Simulation
Screening
Databases
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

これを引用

Baicalein Inhibits Stx1 and 2 of EHE : Effects of Baicalein on the Cytotoxicity, Production, and Secretion of Shiga Toxins of Enterohaemorrhagic Escherichia coli. / Vinh, Pham Thi; Shinohara, Yui; Yamada, Akifumi; Duc, Hoang Minh; Nakayama, Motokazu; Ozawa, Tadahiro; Sato, Jun; Masuda, Yoshimitsu; Honjoh, Ken Ichi; Miyamoto, Takahisa.

:: Toxins, 巻 11, 番号 9, 29.08.2019.

研究成果: ジャーナルへの寄稿記事

Vinh, Pham Thi ; Shinohara, Yui ; Yamada, Akifumi ; Duc, Hoang Minh ; Nakayama, Motokazu ; Ozawa, Tadahiro ; Sato, Jun ; Masuda, Yoshimitsu ; Honjoh, Ken Ichi ; Miyamoto, Takahisa. / Baicalein Inhibits Stx1 and 2 of EHE : Effects of Baicalein on the Cytotoxicity, Production, and Secretion of Shiga Toxins of Enterohaemorrhagic Escherichia coli. :: Toxins. 2019 ; 巻 11, 番号 9.
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abstract = "Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen. Baicalein (5,6,7-trihydroxylflavone), a flavone isolated from the roots of Scutellaria baicalensis, is considered as a potential antibacterial agent to control foodborne pathogens. Among seven compounds selected by in silico screening of the natural compound database, baicalein inhibited the cytotoxicity of both Shiga toxins 1 and 2 (Stx1 and Stx2) against Vero cells after pretreatment at 0.13 mmol/L. In addition, baicalein reduced the susceptibility of Vero cells to both Stx1 and Stx2. Real-time qPCR showed that baicalein increased transcription of stx1 but not of stx2. However, baicalein had no effects on production or secretion of Stx1 or Stx2. Docking models suggested that baicalein formed a stable structure with StxB pentamer with low intramolecular energy. The results demonstrate that inhibitory activity of baicalein against the cytotoxicity of both Stx1 and Stx2 might be due to of the formation of a binding structure inside the pocket of the Stx1B and Stx2B pentamers.",
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T2 - Effects of Baicalein on the Cytotoxicity, Production, and Secretion of Shiga Toxins of Enterohaemorrhagic Escherichia coli

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AU - Shinohara, Yui

AU - Yamada, Akifumi

AU - Duc, Hoang Minh

AU - Nakayama, Motokazu

AU - Ozawa, Tadahiro

AU - Sato, Jun

AU - Masuda, Yoshimitsu

AU - Honjoh, Ken Ichi

AU - Miyamoto, Takahisa

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AB - Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen. Baicalein (5,6,7-trihydroxylflavone), a flavone isolated from the roots of Scutellaria baicalensis, is considered as a potential antibacterial agent to control foodborne pathogens. Among seven compounds selected by in silico screening of the natural compound database, baicalein inhibited the cytotoxicity of both Shiga toxins 1 and 2 (Stx1 and Stx2) against Vero cells after pretreatment at 0.13 mmol/L. In addition, baicalein reduced the susceptibility of Vero cells to both Stx1 and Stx2. Real-time qPCR showed that baicalein increased transcription of stx1 but not of stx2. However, baicalein had no effects on production or secretion of Stx1 or Stx2. Docking models suggested that baicalein formed a stable structure with StxB pentamer with low intramolecular energy. The results demonstrate that inhibitory activity of baicalein against the cytotoxicity of both Stx1 and Stx2 might be due to of the formation of a binding structure inside the pocket of the Stx1B and Stx2B pentamers.

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