Baicalein inhibits Stx1 and 2 of EHEC: Effects of baicalein on the cytotoxicity, production, and secretion of shiga toxins of enterohaemorrhagic escherichia coli

Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Hoang Minh Duc, Motokazu Nakayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken Ichi Honjoh, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen. Baicalein (5,6,7-trihydroxylflavone), a flavone isolated fromthe roots of Scutellaria baicalensis, is considered as a potential antibacterial agent to control foodborne pathogens. Among seven compounds selected by in silico screening of the natural compound database, baicalein inhibited the cytotoxicity of both Shiga toxins 1 and 2 (Stx1 and Stx2) against Vero cells after pretreatment at 0.13 mmol/L. In addition, baicalein reduced the susceptibility of Vero cells to both Stx1 and Stx2. Real-time qPCR showed that baicalein increased transcription of stx1 but not of stx2. However, baicalein had no effects on production or secretion of Stx1 or Stx2. Docking models suggested that baicalein formed a stable structure with StxB pentamer with low intramolecular energy. The results demonstrate that inhibitory activity of baicalein against the cytotoxicity of both Stx1 and Stx2 might be due to of the formation of a binding structure inside the pocket of the Stx1B and Stx2B pentamers.

本文言語英語
論文番号505
ジャーナルToxins
11
9
DOI
出版ステータス出版済み - 8月 29 2019

!!!All Science Journal Classification (ASJC) codes

  • 毒物学
  • 健康、毒物学および変異誘発

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