Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering

Nana Shirakigawa, Takayuki Takei, Hiroyuki Ijima

研究成果: Contribution to journalArticle査読

32 被引用数 (Scopus)

抄録

Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.

本文言語英語
ページ(範囲)740-745
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
116
6
DOI
出版ステータス出版済み - 12 2013

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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