Basophilic differentiation of the human leukemia cell line KU812 upon treatment with interleukin-4

Takashi Hara, Koji Yamada, Hirofumi Tachibana

研究成果: ジャーナルへの寄稿記事

32 引用 (Scopus)

抄録

The human leukemia cell line KU812 had been described as an immature prebasophilic cell line and exhibits a potential to differentiate into mature basophils. We studied the effect of interleukin-4 (IL-4) on the basophilic differentiation of KU812 cells. When KU812 cells were cultured with 1 ng/ml IL-4, cellular histamine content increased more than 10-fold. IL-4 also enhanced the expression of FcεRI, a high affinity IgE receptor, on the cell surface. KU812 cells treated with IL-4 expressed higher levels of FcεRIα, FcεRIβ and FcεRIγ mRNA than non treated KU812 cells. After 21 days in culture with IL-4, KU812 cells became morphologically mature basophilic cells as demonstrated by staining positive for cytoplasmic granules and heparin proteoglycan by Wright dye and toluidin blue dye respectively. In addition, IgE-mediated histamine release was observed, suggesting that the FcεRI induced by IL-4 was functional and was able to transduce a signal for degranulation. These results suggest that IL-4 promotes differentiation of KU812 cells into mature basophilic cells both morphologically and functionally.

元の言語英語
ページ(範囲)542-548
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
247
発行部数3
DOI
出版物ステータス出版済み - 6 29 1998

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Interleukin-4
Leukemia
Cells
Cell Line
Histamine
Cell Differentiation
Coloring Agents
Cytoplasmic Granules
IgE Receptors
Basophils
Histamine Release
Immunoglobulin E
Cultured Cells
Staining and Labeling
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Basophilic differentiation of the human leukemia cell line KU812 upon treatment with interleukin-4. / Hara, Takashi; Yamada, Koji; Tachibana, Hirofumi.

:: Biochemical and Biophysical Research Communications, 巻 247, 番号 3, 29.06.1998, p. 542-548.

研究成果: ジャーナルへの寄稿記事

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