Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells

S. Toujima, K. Kuwano, Y. Zhang, N. Fujimoto, M. Hirama, T. Oishi, S. Fukuda, Y. Nagumo, H. Imai, T. Kikuchi, S. Arai

研究成果: Contribution to journalArticle

6 被引用数 (Scopus)

抄録

A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh-Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O-[2'-O- (α-D-glucopyranosyl)-6'-O-acyl-α-D-glucopyranosyl]-1, 2-di-O-acyl-sn-glycerol (GAGDG) using 1H- and 13C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC14 (17·8%), isoC14 (10·7%) and nC16 (34·9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC14 acyl group was higher than those with nC14 and nC16 acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs.

本文言語英語
ページ(範囲)2317-2323
ページ数7
ジャーナルMicrobiology
146
9
DOI
出版ステータス出版済み - 1 1 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • Microbiology

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