Binding of human mitochondrial transcription factor A, an HMG box protein, to a four-way DNA junction

Tetsuji Ohno, Shuyo Umeda, Naotaka Hamasaki, Dongchon Kang

研究成果: ジャーナルへの寄稿学術誌査読

51 被引用数 (Scopus)

抄録

Mitochondrial transcription factor A (mtTFA), the only known transcription factor in mitochondria, is also implicated in maintenance of mitochondrial genome although little is elucidated about its molecular basis. mtTFA is a member of HMG box proteins family. Some HMG proteins bind with high affinity to four-way DNA junctions that mimic a Holliday structure, a putative intermediate in DNA recombination. To explore possible involvement of a Holliday-like structure in the maintenance of mitochondrial genome, we examine the binding of recombinant human mtTFA to a synthetic four-way DNA junction. The human mtTFA binds to the four-way DNA junction with an approximately 10-fold higher affinity than to the corresponding linear duplex DNA and with essentially the same affinity as to a 40-mer DNA containing the human mitochondrial light strand promoter sequence. The mtTFA binds to the four-way as a monomer. Both of the two HMG box domains of human mtTFA are required for the high affinity binding to the four-way junction. (C) 2000 Academic Press.

本文言語英語
ページ(範囲)492-498
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
271
2
DOI
出版ステータス出版済み - 5月 10 2000

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Binding of human mitochondrial transcription factor A, an HMG box protein, to a four-way DNA junction」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル