Binding of lectins to DNA micro-assemblies: Modification of nucleo-cages with lactose-conjugated psoralen

Kwonil Kim, Kazunori Matsuura, Nobuo Kimizuka

    研究成果: ジャーナルへの寄稿学術誌査読

    23 被引用数 (Scopus)

    抄録

    Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M-1.

    本文言語英語
    ページ(範囲)4311-4317
    ページ数7
    ジャーナルBioorganic and Medicinal Chemistry
    15
    12
    DOI
    出版ステータス出版済み - 6月 15 2007

    !!!All Science Journal Classification (ASJC) codes

    • 生化学
    • 分子医療
    • 分子生物学
    • 薬科学
    • 創薬
    • 臨床生化学
    • 有機化学

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