Binding of the capsule-like serotype-specific polysaccharide antigen and the lipopolysaccharide from Actinobacillus actinomycetemcomitans to human complement-derived opsonins

N. Yamaguchi, H. Tsuda, Yoshihisa Yamashita, T. Koga

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

We investigated the molecular mechanism of resistance of Actinobacillus actinomycetemcomitans to complement-dependent chemiluminescence response by human polymorphonuclear leukocytes. Whole cells of serotype b-specific polysaccharide antigen-defective mutants ST2 and ST5 were constructed by inserting transposon Tn916 into A. actinomycetemcomitans strain Y4. These strains induced strong chemiluminescence response by human polymorphonuclear leukocytes and markedly bound to human complement-derived opsonins. In contrast, strain Y4 induced weak chemiluminescence response and weakly bound to complement-derived opsonins. The biosensor analysis revealed that lipopolysaccharide from strain Y4 strongly bound to human C3b, but serotype b-specific polysaccharide antigen did not. The serotype b-specific polysaccharide antigen molecule might sterically hinder the interaction between complement-derived opsonins and lipopolysaccharide to reduce complement-dependent chemiluminescence response by human polymorphonuclear leukocytes.

本文言語英語
ページ(範囲)348-354
ページ数7
ジャーナルOral Microbiology and Immunology
13
6
DOI
出版ステータス出版済み - 1月 1 1998

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • 歯科学(全般)
  • 微生物学(医療)

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