Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori

Takumi Mitsudome, Hiroaki Mon, Jian Xu, Zhiqing Li, Man Lee, Anandrao Ashok Patil, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

DNA methylation is an important epigenetic mechanism involved in gene expression of vertebrates and invertebrates. In general, DNA methylation profile is established by de novo DNA methyltransferases (DNMT-3A, -3B) and maintainance DNA methyltransferase (DNMT-1). DNMT-1 has a strong substrate preference for hemimethylated DNA over the unmethylated one. Because the silkworm genome lacks an apparent homologue of de novo DNMT, it is still unclear that how silkworm chromosome establishes and maintains its DNA methylation profile. As the first step to unravel this enigma, we purified recombinant BmDNMT-1 using baculovirus expression system and characterized its DNA-binding and DNA methylation activity. We found that the BmDNMT-1 preferentially methylates hemimethylated DNA despite binding to both unmethylated and hemimethylated DNA. Interestingly, BmDNMT-1 formed a complex with DNA in the presence or absence of methyl group donor, S-Adenosylmethionine (AdoMet) and the AdoMet-dependent complex formation was facilitated by Zn2+ and Mn2+. Our results provide clear evidence that BmDNMT-1 retained the function as maintenance DNMT but its sensitivity to metal ions is different from mammalian DNMT-1.

元の言語英語
ページ(範囲)55-65
ページ数11
ジャーナルInsect Biochemistry and Molecular Biology
58
DOI
出版物ステータス出版済み - 3 1 2015

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Bombyx
methyltransferases
Methyltransferases
Bombyx mori
silkworms
DNA Methylation
Maintenance
DNA methylation
DNA
S-Adenosylmethionine
adenosylmethionine
Baculoviridae
Invertebrates
Chromosomes
Epigenomics
Gene expression
Metal ions
Vertebrates
S-adenosylmethionine
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

これを引用

Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori. / Mitsudome, Takumi; Mon, Hiroaki; Xu, Jian; Li, Zhiqing; Lee, Man; Patil, Anandrao Ashok; Masuda, Atsushi; Iiyama, Kazuhiro; Morokuma, Daisuke; Kusakabe, Takahiro.

:: Insect Biochemistry and Molecular Biology, 巻 58, 01.03.2015, p. 55-65.

研究成果: ジャーナルへの寄稿記事

Mitsudome, Takumi ; Mon, Hiroaki ; Xu, Jian ; Li, Zhiqing ; Lee, Man ; Patil, Anandrao Ashok ; Masuda, Atsushi ; Iiyama, Kazuhiro ; Morokuma, Daisuke ; Kusakabe, Takahiro. / Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori. :: Insect Biochemistry and Molecular Biology. 2015 ; 巻 58. pp. 55-65.
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