Biochemical characterization of Pkn2, a protein Ser/Thr kinase from Myxococcus xanthus, a Gram-negative developmental bacterium

Hiroshi Udo, Masayori Inouye, Sumiko Inouye

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Pkn2, a protein Ser/Thr kinase, from the developmental bacterium Myxococcus xanthus was expressed under a T7 promoter in Escherichia coli and purified. Purified Pkn2 retained the autophosphorylation activity with the K(m) value of 177 μM for ATP and 73 nmol/min/mg for V(max). The optimum pH and temperature were determined to be 7.5 and 35°C, respectively. The autophosphorylation activity was inhibited by staurosporine with the IC50 value of 400 nM while H-7 and genistein had little effect on this kinase. Pkn2 appears to be unique for its higher manganese dependence. This is the first biochemical characterization of the prokaryotic protein Ser/Thr kinase.

元の言語英語
ページ(範囲)188-192
ページ数5
ジャーナルFEBS Letters
400
発行部数2
DOI
出版物ステータス出版済み - 1 3 1997

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Myxococcus xanthus
Gram-Negative Bacteria
Bacteria
Phosphotransferases
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Proteins
Staurosporine
Genistein
Manganese
Escherichia coli
Inhibitory Concentration 50
Adenosine Triphosphate
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Biochemical characterization of Pkn2, a protein Ser/Thr kinase from Myxococcus xanthus, a Gram-negative developmental bacterium. / Udo, Hiroshi; Inouye, Masayori; Inouye, Sumiko.

:: FEBS Letters, 巻 400, 番号 2, 03.01.1997, p. 188-192.

研究成果: ジャーナルへの寄稿記事

Udo, Hiroshi ; Inouye, Masayori ; Inouye, Sumiko. / Biochemical characterization of Pkn2, a protein Ser/Thr kinase from Myxococcus xanthus, a Gram-negative developmental bacterium. :: FEBS Letters. 1997 ; 巻 400, 番号 2. pp. 188-192.
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