Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori

Kohji Yamamoto, Sumiharu Nagaoka, Yutaka Banno, Yoichi Aso

研究成果: ジャーナルへの寄稿記事

46 引用 (Scopus)

抄録

A cDNA encoding an omega-class glutathione S-transferase of the silkmoth, Bombyx mori (bmGSTO), was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and deduced for amino acid sequence, which revealed 40, 40, and 39% identities to omega-class GSTs from human, pig, and mouse, respectively. A recombinant protein (rbmGSTO) was functionally overexpressed in Escherichia coli cells in a soluble form and purified to homogeneity. rbmGSTO was able to catalyze the biotranslation of glutathione with 1-chloro-2,4-dinitrobenzene, a model substrate for GST, as well as with 4-hydroxynonenal, a product of lipid peroxidation. This enzyme was shown to have high affinity for organophosphorus insecticide and was present abundantly in silkmoth strain exhibiting fenitrothion resistance. These results indicate that bmGSTO could be involved in the increase in level of insecticide resistance for lepidopteran insects.

元の言語英語
ページ(範囲)461-467
ページ数7
ジャーナルComparative Biochemistry and Physiology - C Toxicology and Pharmacology
149
発行部数4
DOI
出版物ステータス出版済み - 5 1 2009

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Bombyx
Insecticides
Glutathione Transferase
Fenitrothion
Dinitrochlorobenzene
Polymerase chain reaction
RNA-Directed DNA Polymerase
Recombinant Proteins
Insecticide Resistance
Escherichia coli
Glutathione
Complementary DNA
Reverse Transcriptase Polymerase Chain Reaction
Lipids
Amino Acids
Lipid Peroxidation
Insects
Amino Acid Sequence
Substrates
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

これを引用

Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori. / Yamamoto, Kohji; Nagaoka, Sumiharu; Banno, Yutaka; Aso, Yoichi.

:: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 巻 149, 番号 4, 01.05.2009, p. 461-467.

研究成果: ジャーナルへの寄稿記事

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