Optimization of alkaline pretreatment of sugarcane bagasse for consolidated bioprocessing fermentation by the cellulose-fermenting fungus Phlebia sp. MG-60 was studied. The lignin and xylan contents of bagasse were decreased and ethanol production from each pretreated sugarcane bagasse by MG-60 was increased in an alkaline concentration-dependent manner. The fungus produced cellulase and xylanase rapidly over 120h. When this fungus was cultured with 20gL-1 of sugarcane bagasse pretreated with NaOH (0.8wt%, 121°C, 60min), 4.5gL-1 ethanol was produced, equivalent to 210mg ethanol per gram of the original untreated bagasse after 240h fermentation, giving ethanol yields of 65.7% of the theoretical maximum. These data suggest that Phlebia sp. MG-60 is a potential candidate for ethanol production from alkali-pretreated bagasse in a single bioreactor, without enzymatic or chemical hydrolysis.
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