Biological activity of the substrate-induced fibronectin network: Insight into the third dimension through electrospun fibers

Dencho Gugutkov, Cristina González-García, José Carlos Rodríguez Hernández, George Altankov, Manuel Salmerón-Sánchez

研究成果: ジャーナルへの寄稿学術誌査読

46 被引用数 (Scopus)

抄録

Fibronectin (FN) fibrillogenesis is a cell-mediated process involving integrin activation that results in conformational changes of FN molecules and the organization of actin cytoskeleton. A similar process can be induced by some chemistries in the absence of cells, e.g., poly(ethyl acrylate) (PEA), which enhance FN-FN interactions leading to the formation of a biologically active network. Atomic force microscopy images of single FN molecules, at the early stages of adsorption on plane PEA, allow one to rationalize the process. Further, the role of the spatial organization of the FN network on the cellular response is investigated through its adsorption on electrospun fibers. Randomly oriented and aligned PEA fibers were prepared to mimic the three-dimensional organization of the extracellular matrix. The formation of theFNnetwork on the PEA fibers but not on the supporting coverglass was confirmed. Fibroblasts aligned with oriented fibers, displayed extended morphology, developed linearly organized focal adhesion complexes, and matured actin filaments. Conversely, on random PEA fibers, cells acquired polygonal morphology with altered actin cytoskeleton but well-developed focal adhesions. LateFNmatrix formation was also influenced: spatially organizedFN matrix fibrils along the oriented PEA fibers and an altered arrangement on random ones.

本文言語英語
ページ(範囲)10893-10900
ページ数8
ジャーナルLangmuir
25
18
DOI
出版ステータス出版済み - 9月 15 2009
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 凝縮系物理学
  • 表面および界面
  • 分光学
  • 電気化学

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