Biological control of potato tuber soft rot using N-acyl-L-homoserine lactone-degrading endophytic bacteria

Nguyen Thanh Ha, Tran Quang Minh, Pham Xuan Hoi, Nguyen Thi Thanh Thuy, Naruto Furuya, Hoang Hoa Long

研究成果: ジャーナルへの寄稿記事

抄録

Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103-104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.

元の言語英語
ページ(範囲)1921-1927
ページ数7
ジャーナルCurrent Science
115
発行部数10
DOI
出版物ステータス出版済み - 11 1 2018

Fingerprint

homoserine
lactones
tubers
biological control
potatoes
bacteria
Pectobacterium carotovorum
Variovorax paradoxus
Variovorax
Chromobacterium violaceum
quorum sensing
biosensors
maceration
Bacillus (bacteria)
Agrobacterium radiobacter
Vietnam
biological control agents
virulence
pathogens
enzymes

All Science Journal Classification (ASJC) codes

  • General

これを引用

Biological control of potato tuber soft rot using N-acyl-L-homoserine lactone-degrading endophytic bacteria. / Ha, Nguyen Thanh; Minh, Tran Quang; Hoi, Pham Xuan; Thanh Thuy, Nguyen Thi; Furuya, Naruto; Long, Hoang Hoa.

:: Current Science, 巻 115, 番号 10, 01.11.2018, p. 1921-1927.

研究成果: ジャーナルへの寄稿記事

Ha, Nguyen Thanh ; Minh, Tran Quang ; Hoi, Pham Xuan ; Thanh Thuy, Nguyen Thi ; Furuya, Naruto ; Long, Hoang Hoa. / Biological control of potato tuber soft rot using N-acyl-L-homoserine lactone-degrading endophytic bacteria. :: Current Science. 2018 ; 巻 115, 番号 10. pp. 1921-1927.
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abstract = "Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103-104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.",
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