Biological functions of UDP-glucose synthesis in Streptococcus mutans

Yoshihisa Yamashita, Yuichi Tsukioka, Yoshio Nakano, Kiyotaka Tomihisa, Takahiko Oho, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

34 引用 (Scopus)

抄録

A gene encoding glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) was isolated from Streptococcus mutans. A cell extract of Escherichia coli expressing the cloned gene exhibited glucose-l-phosphate uridylyltransferase activity. The enzyme catalyses the conversion of D-glucose 1-phosphate and UTP into UDP-D-glucose. Rabbit antiserum against the serotype-c-specific antigen did not react with autoclaved extracts from mutant cells in which the cloned gene was insertionally inactivated. The glucose content of the cell-wall preparation purified from the mutant was very much lowered, whereas there was no observable decrease in the content of rhamnose. When the mutant strain was grown in an acidic environment, its cell viability was much lower than that of the wild-type. These results suggest that UDP-D-glucose functions not only as an immediate precursor of the serotype-c-specific antigen of S. mutans (as a glucose donor for side-chain formation), but is also important for the organism's viability in environmental conditions of low pH.

元の言語英語
ページ(範囲)1235-1245
ページ数11
ジャーナルMicrobiology
144
発行部数5
DOI
出版物ステータス出版済み - 5 1998

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Uridine Diphosphate Glucose
Streptococcus mutans
Glucose
Cell Extracts
Genes
Antigens
Rhamnose
Uridine Triphosphate
Cell Wall
Immune Sera
Cell Survival
Phosphates
Escherichia coli
Rabbits
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology

これを引用

Yamashita, Y., Tsukioka, Y., Nakano, Y., Tomihisa, K., Oho, T., & Koga, T. (1998). Biological functions of UDP-glucose synthesis in Streptococcus mutans. Microbiology, 144(5), 1235-1245. https://doi.org/10.1099/00221287-144-5-1235

Biological functions of UDP-glucose synthesis in Streptococcus mutans. / Yamashita, Yoshihisa; Tsukioka, Yuichi; Nakano, Yoshio; Tomihisa, Kiyotaka; Oho, Takahiko; Koga, Toshihiko.

:: Microbiology, 巻 144, 番号 5, 05.1998, p. 1235-1245.

研究成果: ジャーナルへの寄稿記事

Yamashita, Y, Tsukioka, Y, Nakano, Y, Tomihisa, K, Oho, T & Koga, T 1998, 'Biological functions of UDP-glucose synthesis in Streptococcus mutans', Microbiology, 巻. 144, 番号 5, pp. 1235-1245. https://doi.org/10.1099/00221287-144-5-1235
Yamashita, Yoshihisa ; Tsukioka, Yuichi ; Nakano, Yoshio ; Tomihisa, Kiyotaka ; Oho, Takahiko ; Koga, Toshihiko. / Biological functions of UDP-glucose synthesis in Streptococcus mutans. :: Microbiology. 1998 ; 巻 144, 番号 5. pp. 1235-1245.
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