Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

Taisuke Shimada, Takao Yasui, Asami Yokoyama, Tatsuro Goda, Mitsuo Hara, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Yuji Miyahara, Tomoji Kawai, Yoshinobu Baba

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抜粋

Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

元の言語英語
ページ(範囲)3225-3229
ページ数5
ジャーナルLab on a Chip
18
発行部数21
DOI
出版物ステータス出版済み - 1 1 2018

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

これを引用

Shimada, T., Yasui, T., Yokoyama, A., Goda, T., Hara, M., Yanagida, T., ... Baba, Y. (2018). Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer. Lab on a Chip, 18(21), 3225-3229. https://doi.org/10.1039/c8lc00438b