TY - JOUR
T1 - Biosynthetic mechanism for L-gulose in main polar lipids of thermoplasma acidophilum and possible resemblance to plant ascorbic acid biosynthesis
AU - Yamauchi, Noriaki
AU - Nakayama, Yusuke
PY - 2013/11/4
Y1 - 2013/11/4
N2 - L-Gulose is a very rare sugar, but appears as a sugar component of the main polar lipids characteristic in such a thermophilic archaeon as Thermoplasma acidophilum that lives without cell walls in a highly acidic environment. The biosynthesis of L-gulose in this thermophilic organism was investigated with deuterium- labeling experiments. L-Gulose was found to be biosynthesized from D-glucose via stepwise stereochemical inversion at C-2 and C-5. The involvement of an epimerase related to GDP-mannose 3,5-epimerase, the key enzyme of plant ascorbate biosynthesis, was also suggested in this C-5 inversion. The resemblance of Lgulose biosynthesis in archaea and plants might be suggested from these results.
AB - L-Gulose is a very rare sugar, but appears as a sugar component of the main polar lipids characteristic in such a thermophilic archaeon as Thermoplasma acidophilum that lives without cell walls in a highly acidic environment. The biosynthesis of L-gulose in this thermophilic organism was investigated with deuterium- labeling experiments. L-Gulose was found to be biosynthesized from D-glucose via stepwise stereochemical inversion at C-2 and C-5. The involvement of an epimerase related to GDP-mannose 3,5-epimerase, the key enzyme of plant ascorbate biosynthesis, was also suggested in this C-5 inversion. The resemblance of Lgulose biosynthesis in archaea and plants might be suggested from these results.
UR - http://www.scopus.com/inward/record.url?scp=84886685770&partnerID=8YFLogxK
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U2 - 10.1271/bbb.130442
DO - 10.1271/bbb.130442
M3 - Article
C2 - 24096672
AN - SCOPUS:84886685770
VL - 77
SP - 2087
EP - 2093
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -