Biotinylation of silicon and nickel surfaces and detection of streptavidin as biosensor

Hirokazu Seto, Chie Yamashita, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Mitsuhiro Matsuno, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol-ene click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin-streptavidin-biotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.

本文言語英語
ページ(範囲)9457-9463
ページ数7
ジャーナルLangmuir
29
30
DOI
出版ステータス出版済み - 7 30 2013

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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