Biphasic and directed translocation of protein kinase Cα inside cultured endothelial cells before migration

Masataka Arai, Tomoya Shimada, Chihiro Kora, Kazuhiro Nakashima, Toshihiro Sera, Susumu Kudo

    研究成果: Contribution to journalArticle査読

    5 被引用数 (Scopus)


    Mechanical wounding of an endothelial monolayer induces an immediate Ca2+ wave. Several hours later, the denuded area is covered by endothelial cells (ECs) that migrate to the wound. This migration process is closely related to protein kinase Cα (PKCα), a Ca2+-dependent protein that translocates from the cytosol to the cell membrane. Because the cells adjacent to the wounded area are the first to migrate into the wound, we investigated whether a mechanical wound immediately induces PKCα translocation in adjacent cells. We monitored Ca2+ dynamics and PKCα translocation simultaneously using fluorescent microscopy. For this simultaneous observation, we used Fura-2–acetoxymethyl ester to visualize Ca2+ and constructed a green fluorescent protein-tagged fusion protein to visualize PKCα. Mechanical wounding of the endothelial monolayer induced an immediate Ca2+ wave in cells adjacent to the wounded cells before their migration. Almost concurrently, PKCα in the neighboring cells translocated to the cell membrane, then accumulated at the periphery near the wounded cell. This report is the first description of this biphasic and directed translocation of PKCα in cells before cell migration. Our results may provide new insights into the directed migration of ECs.

    ジャーナルBiochemistry and Biophysics Reports
    出版ステータス出版済み - 12 2017

    All Science Journal Classification (ASJC) codes

    • 生物理学
    • 生化学


    「Biphasic and directed translocation of protein kinase Cα inside cultured endothelial cells before migration」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。