Bisphenol A-induced apoptosis of cultured rat Sertoli cells

Hiroshi Iida, Kazue Maehara, Masamichi Doiguchi, Takayuki Mõri, Fumio Yamada

研究成果: ジャーナルへの寄稿学術誌査読

73 被引用数 (Scopus)

抄録

Bisphenol A (BPA) was examined for its effects on cultured Sertoli cells established from 18-day-old rat testes. We demonstrated that exposure of cultured Sertoli cells to BPA decreased the cell viability in a dose- and a time-dependent manner and that exposure to BPA brought about morphologic changes of the cells, such as membrane blebs, cell rounding, cytoskeletal collapse, and chromatin condensation or fragmentation, all of which conform to the morphologic criteria for apoptosis. Immunocytochemistry showed that active caspase-3, a major execution caspase, was expressed in round Sertoli cells positively labeled by the TUNEL method. Co-localization of active caspase-3 and aggregated actin fragments was also observed in the round Sertoli cells. Theses results suggest that BPA induces cell death of Sertoli cells by promoting apoptosis. Apoptosis-inducing cell death was observed in cells exposed to 150-200 μM BPA, while BPA at < 100 μM had only slight cytotoxic effects on the cells.

本文言語英語
ページ(範囲)457-464
ページ数8
ジャーナルReproductive Toxicology
17
4
DOI
出版ステータス出版済み - 2003

!!!All Science Journal Classification (ASJC) codes

  • 毒物学

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