Block versus random amphiphilic copolymers as antibacterial agents

Yukari Oda, Shokyoku Kanaoka, Takahiro Sato, Sadahito Aoshima, Kenichi Kuroda

研究成果: ジャーナルへの寄稿記事

114 引用 (Scopus)

抄録

We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against Escherichia coli. However, the block copolymers are much less hemolytic compared to the highly hemolytic random copolymers. These results indicate that the amphiphilic copolymer structure is a key determinant of activity. Furthermore, the block copolymers induced dye leakage from lipid vesicles consisting of E. coli-type lipids, but not mammalian lipids, while the random copolymers disrupted both types of vesicles. In addition, both copolymers displayed bactericidal and hemolytic activities at concentrations 1 or 2 orders of magnitude lower than their critical (intermolecular) aggregation concentrations (CACs), as determined by light scattering measurements. This suggests that polymer aggregation or macromolecular assembly is not a requisite for the antibacterial activity and selectivity against bacteria over human red blood cells (RBCs). We speculate that different single-chain conformations between the block and random copolymers play an important role in the antibacterial action and underlying antibacterial mechanisms.

元の言語英語
ページ(範囲)3581-3591
ページ数11
ジャーナルBiomacromolecules
12
発行部数10
DOI
出版物ステータス出版済み - 10 10 2011
外部発表Yes

Fingerprint

Bactericides
Copolymers
Anti-Bacterial Agents
Lipids
Escherichia coli
Block copolymers
Agglomeration
Cationic polymerization
Living polymerization
Light scattering
Conformations
Ethers
Bacteria
Polymers
Blood
Coloring Agents
Dyes
Monomers
Cells
Derivatives

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

これを引用

Oda, Y., Kanaoka, S., Sato, T., Aoshima, S., & Kuroda, K. (2011). Block versus random amphiphilic copolymers as antibacterial agents. Biomacromolecules, 12(10), 3581-3591. https://doi.org/10.1021/bm200780r

Block versus random amphiphilic copolymers as antibacterial agents. / Oda, Yukari; Kanaoka, Shokyoku; Sato, Takahiro; Aoshima, Sadahito; Kuroda, Kenichi.

:: Biomacromolecules, 巻 12, 番号 10, 10.10.2011, p. 3581-3591.

研究成果: ジャーナルへの寄稿記事

Oda, Y, Kanaoka, S, Sato, T, Aoshima, S & Kuroda, K 2011, 'Block versus random amphiphilic copolymers as antibacterial agents', Biomacromolecules, 巻. 12, 番号 10, pp. 3581-3591. https://doi.org/10.1021/bm200780r
Oda, Yukari ; Kanaoka, Shokyoku ; Sato, Takahiro ; Aoshima, Sadahito ; Kuroda, Kenichi. / Block versus random amphiphilic copolymers as antibacterial agents. :: Biomacromolecules. 2011 ; 巻 12, 番号 10. pp. 3581-3591.
@article{d1efd70802404e6d9e8c2041f745e0c5,
title = "Block versus random amphiphilic copolymers as antibacterial agents",
abstract = "We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against Escherichia coli. However, the block copolymers are much less hemolytic compared to the highly hemolytic random copolymers. These results indicate that the amphiphilic copolymer structure is a key determinant of activity. Furthermore, the block copolymers induced dye leakage from lipid vesicles consisting of E. coli-type lipids, but not mammalian lipids, while the random copolymers disrupted both types of vesicles. In addition, both copolymers displayed bactericidal and hemolytic activities at concentrations 1 or 2 orders of magnitude lower than their critical (intermolecular) aggregation concentrations (CACs), as determined by light scattering measurements. This suggests that polymer aggregation or macromolecular assembly is not a requisite for the antibacterial activity and selectivity against bacteria over human red blood cells (RBCs). We speculate that different single-chain conformations between the block and random copolymers play an important role in the antibacterial action and underlying antibacterial mechanisms.",
author = "Yukari Oda and Shokyoku Kanaoka and Takahiro Sato and Sadahito Aoshima and Kenichi Kuroda",
year = "2011",
month = "10",
day = "10",
doi = "10.1021/bm200780r",
language = "English",
volume = "12",
pages = "3581--3591",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Block versus random amphiphilic copolymers as antibacterial agents

AU - Oda, Yukari

AU - Kanaoka, Shokyoku

AU - Sato, Takahiro

AU - Aoshima, Sadahito

AU - Kuroda, Kenichi

PY - 2011/10/10

Y1 - 2011/10/10

N2 - We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against Escherichia coli. However, the block copolymers are much less hemolytic compared to the highly hemolytic random copolymers. These results indicate that the amphiphilic copolymer structure is a key determinant of activity. Furthermore, the block copolymers induced dye leakage from lipid vesicles consisting of E. coli-type lipids, but not mammalian lipids, while the random copolymers disrupted both types of vesicles. In addition, both copolymers displayed bactericidal and hemolytic activities at concentrations 1 or 2 orders of magnitude lower than their critical (intermolecular) aggregation concentrations (CACs), as determined by light scattering measurements. This suggests that polymer aggregation or macromolecular assembly is not a requisite for the antibacterial activity and selectivity against bacteria over human red blood cells (RBCs). We speculate that different single-chain conformations between the block and random copolymers play an important role in the antibacterial action and underlying antibacterial mechanisms.

AB - We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against Escherichia coli. However, the block copolymers are much less hemolytic compared to the highly hemolytic random copolymers. These results indicate that the amphiphilic copolymer structure is a key determinant of activity. Furthermore, the block copolymers induced dye leakage from lipid vesicles consisting of E. coli-type lipids, but not mammalian lipids, while the random copolymers disrupted both types of vesicles. In addition, both copolymers displayed bactericidal and hemolytic activities at concentrations 1 or 2 orders of magnitude lower than their critical (intermolecular) aggregation concentrations (CACs), as determined by light scattering measurements. This suggests that polymer aggregation or macromolecular assembly is not a requisite for the antibacterial activity and selectivity against bacteria over human red blood cells (RBCs). We speculate that different single-chain conformations between the block and random copolymers play an important role in the antibacterial action and underlying antibacterial mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=80053955350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053955350&partnerID=8YFLogxK

U2 - 10.1021/bm200780r

DO - 10.1021/bm200780r

M3 - Article

C2 - 21846110

AN - SCOPUS:80053955350

VL - 12

SP - 3581

EP - 3591

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 10

ER -